The frequency of toll-like receptor 4 gene polymorphism in ankylosing spondylitis and its relationship between disease activity

Objectives. We aimed to evaluate the frequency of toll-like receptor 4 ( TLR4) gene polymorphism and its relationship between disease activity in patients with ankylosing spondylitis (AS). Methods. Forty-one AS patients (25 male/16 female) fullfilling the 1984 Modified New York Criteria and 41 healthy controls ( 25 male/16 female) were included in this study. Disease activity of the AS patients was assesed by Bath Ankylosing Spondylitis Disease Activity Index (BASDAI). The TLR4 gene polymorphism of AS patients and healthy controls were analyzed by Real-Time Polymerase Chain Reaction (PCR) System. Results. Three (7.3%) patients with AS had TLR4 gene polymorphism compared with healthy controls (0/41; 0%). Two of these patients had heterozygous mutation and one had homozygous mutation. Significant correlation was not found between TLR4 gene polymorphism and BASDAI score ( p > 0.05). Conclusions. In our study, TLR4 gene polymorphism was higher in patients with AS compared with control group. But, this polymorphism was not associated with disease activity, erythrocyte sedimentation rate and C-reactive protein levels. 

[1]  I. Briceño,et al.  The Frequency of HLA-B27 in a Colombian Population with Signs of Spondyloarthritis. , 2017, Current rheumatology reviews.

[2]  D. Baeten,et al.  Serum IgA Immune Complexes Promote Proinflammatory Cytokine Production by Human Macrophages, Monocytes, and Kupffer Cells through FcαRI–TLR Cross-Talk , 2017, The Journal of Immunology.

[3]  M. Borawska,et al.  Concentration of selenium, zinc, copper, Cu/Zn ratio, total antioxidant status and c-reactive protein in the serum of patients with psoriasis treated by narrow-band ultraviolet B phototherapy: A case-control study. , 2017, Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements.

[4]  Zheng Zhang,et al.  Human innate responses and adjuvant activity of TLR ligands in vivo in mice reconstituted with a human immune system. , 2017, Vaccine.

[5]  A. Kratz,et al.  ICSH recommendations for modified and alternate methods measuring the erythrocyte sedimentation rate , 2017, International journal of laboratory hematology.

[6]  E. Tinazzi,et al.  Gene Expression Analysis before and after Treatment with Adalimumab in Patients with Ankylosing Spondylitis Identifies Molecular Pathways Associated with Response to Therapy , 2017, Genes.

[7]  M. Mahmoudi,et al.  New insights toward the pathogenesis of ankylosing spondylitis; genetic variations and epigenetic modifications , 2017, Modern rheumatology.

[8]  V. Calvo,et al.  Comparison of methods for circulating cell-free DNA isolation using blood from cancer patients: impact on biomarker testing. , 2016, Translational lung cancer research.

[9]  Zhiming Lin,et al.  Kunxian capsules in the treatment of patients with ankylosing spondylitis: a randomized placebo-controlled clinical trial , 2016, Trials.

[10]  M. Mahmoudi,et al.  Gene Expression Profiling of Toll-Like Receptor 4 and 5 in Peripheral Blood Mononuclear Cells in Rheumatic Disorders: Ankylosing Spondylitis and Rheumatoid Arthritis. , 2016, Iranian journal of allergy, asthma, and immunology.

[11]  A. Amini,et al.  Erythrocyte Sedimentation Rate Measurement Using as a Rapid Alternative to the Westergren Method , 2015, Emergency.

[12]  A. Akbal,et al.  C-reactive protein gene and Toll-like receptor 4 gene polymorphisms can relate to the development of psoriatic arthritis , 2015, Clinical Rheumatology.

[13]  D. Ye,et al.  Lack of association of TLR4 polymorphisms with susceptibility to rheumatoid arthritis and ankylosing spondylitis: a meta-analysis. , 2012, Joint, bone, spine : revue du rhumatisme.

[14]  M. Mayes,et al.  Whole-blood Gene Expression Profiling in Ankylosing Spondylitis Shows Upregulation of Toll-like Receptor 4 and 5 , 2011, The Journal of Rheumatology.

[15]  L. Bradbury,et al.  Toll-like receptor 4 and CD14 polymorphisms in ankylosing spondylitis: evidence of a weak association in Finns. , 2008, The Journal of rheumatology.

[16]  P. Rahman,et al.  Analysis of single nucleotide polymorphisms in Toll-like receptor 4 shows no association with ankylosing spondylitis in a Korean population , 2008, Rheumatology International.

[17]  L-Z Zhang,et al.  Increased expression of Toll-like receptor 4 in peripheral blood leucocytes and serum levels of some cytokines in patients with ankylosing spondylitis. , 2007 .

[18]  C. Greenwood,et al.  Association of toll-like receptor 4 variants and ankylosing spondylitis: a case-control study. , 2007, The Journal of rheumatology.

[19]  G. Poór,et al.  Lack of genetic association of the Toll-like receptor 4 (TLR4) Asp299Gly and Thr399Ile polymorphisms with spondylarthropathies in a Hungarian population. , 2006, Rheumatology.

[20]  J. Gracie,et al.  TLR4 mutations (Asp299Gly and Thr399Ile) are not associated with ankylosing spondylitis , 2006, Annals of the rheumatic diseases.

[21]  Dominique Baeten,et al.  Tumor necrosis factor alpha blockade treatment down-modulates the increased systemic and local expression of Toll-like receptor 2 and Toll-like receptor 4 in spondylarthropathy. , 2005, Arthritis and rheumatism.

[22]  B. Dijkmans,et al.  No evidence for involvement of the Toll-like receptor 4 (TLR4) A896G and CD14-C260T polymorphisms in susceptibility to ankylosing spondylitis , 2005, Annals of the rheumatic diseases.

[23]  D. Baeten,et al.  Increased systemic and local expression of toll-like receptor 2 and 4 in spondyloarthropathy is downmodulated by tumour necrosis factor alpha blockade , 2005 .