Diffuse backscattering Mueller matricesof highly scattering media.

We report on the development of a method that records spatially dependent intensity patterns of polarized light that is diffusely backscattered from highly scattering media. It is demonstrated that these intensity patterns can be used to differentiate turbid media, such as polystyrene-sphere and biological-cell suspensions. Our technique employs polarized light from a He-Ne laser (l=543nm), which is focused onto the surface of the scattering medium. A surface area of approximately 4x4 cm centered on the light input point is imaged through polarization-analysis optics onto a CCD camera. One can observe a large variety of intensity patterns by varying the polarization state of the incident laser light and changing the analyzer configuration to detect different polarization components of the backscattered light. Introducing the Mueller-matrix concept for diffusely backscattered light, a framework is provided to select a subset of measurements that comprehensively describe the optical properties of backscattering media.

[1]  Jody T. Bruulsema,et al.  Correlation between blood glucose concentration in diabetics and noninvasively measured tissue optical scattering coefficient. , 1997, Optics letters.

[2]  B. Wilson,et al.  A diffusion theory model of spatially resolved, steady-state diffuse reflectance for the noninvasive determination of tissue optical properties in vivo. , 1992, Medical physics.

[3]  M. Nichols,et al.  Design and testing of a white-light, steady-state diffuse reflectance spectrometer for determination of optical properties of highly scattering systems. , 1997, Applied optics.

[4]  William S. Bickel,et al.  The light‐scattering Mueller matrix elements for Rayleigh, Rayleigh–Gans, and Mie spheres , 1987 .

[5]  Irving J. Bigio,et al.  Diffuse polarization spectroscopy on tissue phantoms and biological cell suspensions , 1996, European Conference on Biomedical Optics.

[6]  Steven L. Jacques,et al.  Polarized light transmission through skin using video reflectometry: toward optical tomography of superficial tissue layers , 1996, Photonics West.

[7]  M. Patterson,et al.  Improved solutions of the steady-state and the time-resolved diffusion equations for reflectance from a semi-infinite turbid medium. , 1997, Journal of the Optical Society of America. A, Optics, image science, and vision.

[8]  R. Alfano,et al.  Optical polarization imaging. , 1997, Applied optics.

[9]  S. Jacques,et al.  Hybrid model of Monte Carlo simulation and diffusion theory for light reflectance by turbid media. , 1993, Journal of the Optical Society of America. A, Optics, image science, and vision.

[10]  R. Steiner,et al.  Spatially resolved absolute diffuse reflectance measurements for noninvasive determination of the optical scattering and absorption coefficients of biological tissue. , 1996, Applied optics.

[11]  J. Schmitt,et al.  Use of polarized light to discriminate short-path photons in a multiply scattering medium. , 1992, Applied optics.

[12]  P. Monnier,et al.  Clinical determination of tissue optical properties by endoscopic spatially resolved reflectometry. , 1996, Applied optics.

[13]  A H Hielscher,et al.  Influence of particle size and concentration on the diffuse backscattering of polarized light from tissue phantoms and biological cell suspensions. , 1997, Applied optics.

[14]  J. Mourant,et al.  Predictions and measurements of scattering and absorption over broad wavelength ranges in tissue phantoms. , 1997, Applied optics.

[15]  R. Anderson.  Polarized light examination and photography of the skin. , 1991, Archives of dermatology.

[16]  L. Kunz-Schughart,et al.  Three‐dimensional cell culture induces novel proliferative and metabolic alterations associated with oncogenic transformation , 1996, International journal of cancer.

[17]  M S Patterson,et al.  The use of a neural network to determine tissue optical properties from spatially resolved diffuse reflectance measurements. , 1992, Physics in medicine and biology.