Mutations affecting cysteine synthesis in Aspergillus nidulans : characterization and chromosome mapping

Selection and mapping of mutations affecting cysteine synthesis in Aspergillus nidulans was carried out. A new locus, cysE , is described, the mutants of which are deficient in in vivo conversion of O-acetylserine to cysteine, a step mediated by cysteine synthase. Three loci ( cysB, C and E ) were thus found to control this step in vivo , apparently without affecting the enzyme activity in vitro . By scoring for propargylglycine sensitivity of cys mutants, chromosomal map positions were obtained for all five cysteine loci ( A, B, C, D and E ).

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