DNA preparation and efficient microsatellite analysis from insect hemolymph

A simple and time‐saving method for DNA preparation for efficient microsatellite analysis is described. The method is based on thermal treatment of only 1–5 μL of insect hemolymph in a Chelex 100‐suspension. Since hemolymph withdrawal does not harm the insects, analysis of mating systems, population structure and phylogenetic reconstruction can be conducted with minimal experimental influence.