Some recent advances in the molecular characterization of Echinococcus and Taenia solium.

Some recently obtained data from our laboratory on the molecular characterization of Echinococcus and Taenia solium are described and are complimented by relevant new information obtained by other groups. Progress made in the development of satisfactory immunodiagnostic assays and in the production of recombinant molecules, suitable for application in serology of hydatid disease and cysticercosis, is highlighted. Results arising from the application of polymerase chain reaction and direct sequencing, using primers homologous to evolutionarily conserved sequences, in phylogenetic studies and for distinguishing individual taeniid species are also discussed.

[1]  J. Zhu,et al.  Characterization of taeniid cestodes by DNA analysis. , 1991, Chinese medical journal.

[2]  D. McManus,et al.  The diagnostic value and molecular characterisation of an Echinococcus multilocularis antigen gene clone. , 1991, Molecular and biochemical parasitology.

[3]  D. McManus,et al.  A protein secreted in vivo by Echinococcus granulosus inhibits elastase activity and neutrophil chemotaxis. , 1991, Molecular and biochemical parasitology.

[4]  M. Lightowlers Immunology and molecular biology of Echinococcus infections. , 1990, International journal for parasitology.

[5]  D. McManus Molecular technology: improving strategies for controlling hydatid disease and cysticercosis. , 1990, The Southeast Asian journal of tropical medicine and public health.

[6]  M. Lightowlers Cestode infections in animals: immunological diagnosis and vaccination. , 1990, Revue scientifique et technique.

[7]  A. Flisser,et al.  New approaches in the diagnosis of Taenia solium cysticercosis and taeniasis. , 1990, Annales de parasitologie humaine et comparee.

[8]  M. Lightowlers,et al.  Subunit composition and specificity of the major cyst fluid antigens of Echinococcus granulosus. , 1989, Molecular and biochemical parasitology.

[9]  M. Lightowlers,et al.  Amino acid sequence homology between cyclophilin and a cDNA-cloned antigen of Echinococcus granulosus. , 1989, Molecular and biochemical parasitology.

[10]  N. Müller,et al.  Application of a recombinant Echinococcus multilocularis antigen in an enzyme-linked immunosorbent assay for immunodiagnosis of human alveolar echinococcosis. , 1989, Molecular and biochemical parasitology.

[11]  D. McManus,et al.  The isolation, by differential antibody screening, of Echinococcus multilocularis antigen gene clones with potential for immunodiagnosis. , 1989, Molecular and biochemical parasitology.

[12]  N. Müller,et al.  Plasmid vector for overproduction and export of recombinant protein in Escherichia coli: efficient one-step purification of a recombinant antigen from Echinococcus multilocularis (Cestoda). , 1989, Gene.

[13]  J. D. Smyth,et al.  The physiology and biochemistry of cestodes: The adult cestode: special structural features relevant to its physiology , 1989 .

[14]  J. A. Brand,et al.  An enzyme-linked immunoelectrotransfer blot assay and glycoprotein antigens for diagnosing human cysticercosis (Taenia solium). , 1989, The Journal of infectious diseases.

[15]  N. Müller,et al.  Production of a recombinant antigen of Echinococcus multilocularis with high immunodiagnostic sensitivity and specificity. , 1988, Molecular and biochemical parasitology.

[16]  D. Smith,et al.  Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. , 1988, Gene.

[17]  P. Fan Taiwan taenia and taeniasis. , 1988, Parasitology today.

[18]  C. Lengeler,et al.  Sero-epidemiological survey for alveolar echinococcosis (by Em2-ELISA) of blood donors in an endemic area of Switzerland. , 1987, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[19]  D. McManus,et al.  Specific and cross-reactive antigens of Echinococcus granulosus hydatid cyst fluid. , 1987, Molecular and biochemical parasitology.