Volume reconstruction of the human cornea with oscillating focus scans

For corneal confocal microscopy (CCM) we use the Heidelberg Retina Tomograph (HRT) and the Rostock Cornea Module (RCM; both Heidelberg Engineering GmbH, Heidelberg, Germany). A modification of the HRT software enables the recording of focal image stacks by continually oscillating the focus depth within up to 80 μm. To align the CCM images for volume reconstruction we developed special purpose image registration algorithms, which also correct the motion induced distortions in the CCM images.