Lack of a Fusion Requirement for Development of Bone Marrow-Derived Epithelia

Analysis of developmental plasticity of bone marrow–derived cells (BMDCs) is complicated by the possibility of cell-cell fusion. Here we demonstrate that epithelial cells can develop from BMDCs without cell-cell fusion. We use the Cre/lox system together with β-galactosidase and enhanced green fluorescent protein expression in transgenic mice to identify epithelial cells in the lung, liver, and skin that develop from BMDCs without cell fusion.

[1]  R. McTaggart,et al.  An uncomfortable silence … while we all search for a better reporter gene in adult stem cell biology , 2004 .

[2]  M. Goodell,et al.  Skeletal Muscle Fiber‐Specific Green Autofluorescence: Potential for Stem Cell Engraftment Artifacts , 2004, Stem cells.

[3]  D. Krause,et al.  Plasticity of marrow-derived stem cells. , 2003, Blood.

[4]  Klaus Pfeffer,et al.  Fusion of bone-marrow-derived cells with Purkinje neurons, cardiomyocytes and hepatocytes , 2003, Nature.

[5]  Helen M. Blau,et al.  Stable reprogrammed heterokaryons form spontaneously in Purkinje neurons after bone marrow transplant , 2003, Nature Cell Biology.

[6]  Michael D. Schneider,et al.  Cardiac progenitor cells from adult myocardium: Homing, differentiation, and fusion after infarction , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[7]  G. Vassilopoulos,et al.  Transplanted bone marrow regenerates liver by cell fusion , 2003, Nature.

[8]  M. Grompe,et al.  Cell fusion is the principal source of bone-marrow-derived hepatocytes , 2003, Nature.

[9]  D. Prockop,et al.  Differentiation, cell fusion, and nuclear fusion during ex vivo repair of epithelium by human adult stem cells from bone marrow stroma , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[10]  Helen M. Blau,et al.  Biological Progression from Adult Bone Marrow to Mononucleate Muscle Stem Cell to Multinucleate Muscle Fiber in Response to Injury , 2002, Cell.

[11]  S. Kochanek,et al.  Efficient in vitro and in vivo excision of floxed sequences with a high‐capacity adenoviral vector expressing CRE recombinase , 2002, Genesis.

[12]  E. Scott,et al.  Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion , 2002, Nature.

[13]  Qi-Long Ying,et al.  Changing potency by spontaneous fusion , 2002, Nature.

[14]  T. Hughes,et al.  Cre stoplight: a red/green fluorescent reporter of Cre recombinase expression in living cells. , 2001, BioTechniques.

[15]  Caiying Guo,et al.  Z/EG, a double reporter mouse line that expresses enhanced green fluorescent protein upon cre‐mediated excision , 2000, Genesis.

[16]  J. Morgan,et al.  Evidence for a myogenic stem cell that is exhausted in dystrophic muscle. , 2000, Journal of cell science.

[17]  O. Ciccarelli,et al.  Recurrent postinfantile syncytial giant cell hepatitis after orthotopic liver transplantation , 1998, Transplant international : official journal of the European Society for Organ Transplantation.

[18]  R. Tsien,et al.  green fluorescent protein , 2020, Catalysis from A to Z.

[19]  B. Barraviera,et al.  Liver dysfunction in patients bitten by Crotalus Durissus terrificus (Laurenti, 1768) snakes in Botucatu (State of São Paulo, Brazil). , 1995, Revista do Instituto de Medicina Tropical de Sao Paulo.

[20]  B. Barraviera,et al.  A retrospective study of 40 victims of crotalus snake bites. Analysis of the hepatic necrosis observed in one patient. , 1989, Revista da Sociedade Brasileira de Medicina Tropical.

[21]  J. Hicks,et al.  Neonatal syncytial giant cell hepatitis with paramyxoviral-like inclusions. , 2001, Ultrastructural pathology.

[22]  G. Martin,et al.  Analysis of Fgf8 gene function in vertebrate development. , 1997, Cold Spring Harbor symposia on quantitative biology.