A novel method for discrimination of beef and horsemeat using Raman spectroscopy.

A new approach, based on the usage of Raman spectroscopy in combination with chemometrics, was developed for the rapid determination of beef adulteration with horsemeat. The data mining process of collected Raman spectra was performed with principal component analysis (PCA). Pure fat samples, extracted from forty-nine meat beef and horsemeat samples, were analysed using the Raman spectroscopy. All meat samples were classified successfully according to their origins. The presence of different concentrations (25%, 50%, 75%, w/w) of horsemeat in beef was also differentiated using the developed model system. This study offers a rapid assay for determination of meat adulteration by discriminating beef and horsemeat with high accuracy, a short analysis time (30s) and no requirement for time-consuming sample preparation procedures.

[1]  B. Al-Safadi,et al.  Identification of meat species by PCR-RFLP of the mitochondrial COI gene. , 2012, Meat science.

[2]  V. Segtnan,et al.  The potential of Raman spectroscopy for characterisation of the fatty acid unsaturation of salmon. , 2006, Analytica chimica acta.

[3]  Rafael Jiménez-Flores,et al.  Composition of bovine milk fat globules by confocal Raman microscopy , 2011 .

[4]  Isabel Mafra,et al.  A SYBR Green real-time PCR assay to detect and quantify pork meat in processed poultry meat products. , 2013, Meat science.

[5]  Brian J. Marquardt,et al.  Raman analysis of fish: a potential method for rapid quality screening , 2004 .

[6]  David Lee Phillips,et al.  Raman spectroscopic study of deamidated food proteins , 2009 .

[7]  A. S. Rohman,et al.  Analysis of pork adulteration in beef meatball using Fourier transform infrared (FTIR) spectroscopy. , 2011, Meat science.

[8]  M. Uddin,et al.  Analysis of pork adulteration in commercial meatballs targeting porcine-specific mitochondrial cytochrome b gene by TaqMan probe real-time polymerase chain reaction. , 2012, Meat science.

[9]  F. Martuzzi,et al.  Characteristics of horse meat consumption and production in Italy , 2001 .

[10]  J. Pierna,et al.  Assessment of the discrimination of animal fat by FT-Raman spectroscopy , 2009 .

[11]  Pinkie Jacob Eravuchira,et al.  Fast determination of milk fat content using Raman spectroscopy , 2011 .

[12]  Ioannis Ganopoulos,et al.  A fast and accurate method for controlling the correct labeling of products containing buffalo meat using High Resolution Melting (HRM) analysis. , 2013, Meat science.

[13]  S. Lanteri,et al.  Detection of minced beef adulteration with turkey meat by UV-vis, NIR and MIR spectroscopy , 2013 .

[14]  C. Man.  FTIR spectroscopy combined with chemometrics for analysis of lard in the mixtures with body fats of lamb, cow, and chicken , 2010 .

[15]  Chi Zhang.  Semi-nested multiplex PCR enhanced method sensitivity of species detection in further-processed meats , 2013 .

[16]  Carmen Gallo,et al.  Identification of cattle, llama and horse meat by near infrared reflectance or transflectance spectroscopy. , 2012, Meat science.

[17]  Ali Topcu,et al.  Rapid analysis of sugars in honey by processing Raman spectrum using chemometric methods and artificial neural networks. , 2013, Food chemistry.

[18]  N. Dean,et al.  Using unlabelled data to update classification rules with applications in food authenticity studies , 2006 .

[19]  A. Tiwari,et al.  Beef specific polymerase chain reaction assay for authentication of meat and meat products , 2012 .

[20]  M. Mirghani,et al.  Determination of Lard in Mixture of Body Fats of Mutton and Cow by Fourier Transform Infrared Spectroscopy , 2003 .

[21]  Ana M. Herrero,et al.  Raman spectroscopy a promising technique for quality assessment of meat and fish : A review , 2008 .

[22]  Vincent Baeten,et al.  Oil and Fat Classification by FT-Raman Spectroscopy , 1998 .

[23]  I. Boyaci,et al.  A novel method for quantification of ethanol and methanol in distilled alcoholic beverages using Raman spectroscopy , 2012 .

[24]  M. Mirghani,et al.  Detection of lard mixed with body fats of chicken, lamb, and cow by fourier transform infrared spectroscopy , 2001 .