Inactivation of Vibrio parahaemolyticus in shucked raw oyster (Grassostrea gigas) and clam (Venerupis phillippinarum) by using a combination of NaClO and gamma irradiation

This study investigated the synergistic effects of sodium hypochlorite (NaClO) and gamma irradiation combination against Vibrio parahaemolyticus in shucked oysters and clams. V. parahaemolyticus decreased to 1.1–5.6 log10 CFU/g in oysters and 1.1–5.7 log10CFU/g in clams by NaClO (20–80 ppm) + γ irradiation (0.1–2.0 kGy) combinations. V. parahaemolyticus was not detected by 60 or 80 ppm NaClO + 2.0 kGy. Synergistic reduction of >1 log was observed by 60 ppm NaClO + 0.3–2 kGy and 80 ppm NaClO + 0.5 or 2 kGy. Specifically, >2 log of the synergistic reduction was obtained by 60 or 80 ppm NaClO + 2 kGy. Furthermore, using the Weibull model, 5D values (5-log reductions) were calculated for 60 or 80 ppm NaClO + 0.5–0.9 kGy. No significant differences were observed for all sensory parameters between samples of 2.0 kGy + 0–80 ppm NaClO. This study suggests that 60 ppm NaClO + 2.0 kGy in reducing 7-log V. parahaemolyticus without any deteriorative changes of sensory qualities could be a potential strategy for post-harvest process in seafood processing and distribution to enhance the microbial safety of molluscan shellfish.

[1]  L. Kozačinski,et al.  Microbiological quality of marketed fresh and frozen seafood caught off the Adriatic coast of Croatia , 2018 .

[2]  L. Leibovici,et al.  Systematic Review and Meta-Analysis of In Vitro Synergy of Polymyxins and Carbapenems , 2013, Antimicrobial Agents and Chemotherapy.

[3]  M. Karwe,et al.  Inactivation of Vibrio parahaemolyticus in hard clams (Mercanaria mercanaria) by high hydrostatic pressure (HHP) and the effect of HHP on the physical characteristics of hard clam meat. , 2013, Journal of food science.

[4]  S. Ha,et al.  The synergistic effects of combined NaOCl, gamma irradiation and vitamin B1 on populations of Aeromonas hydrophila in squid , 2012 .

[5]  D. Vugia,et al.  Increasing rates of vibriosis in the United States, 1996-2010: review of surveillance data from 2 systems. , 2012, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[6]  Juyun Lim,et al.  Hedonic scaling: A review of methods and theory , 2011 .

[7]  Jiyong Park,et al.  Effects of combined treatment of sodium hypochlorite/ionizing radiation and addition of vitamin B(1) on microbial flora of oyster and short-necked clam. , 2011, Foodborne pathogens and disease.

[8]  S. Ha,et al.  Synergistic effects of ethanol and UV radiation to reduce levels of selected foodborne pathogenic bacteria. , 2010, Journal of food protection.

[9]  Xianming Shi,et al.  Retention of Vibrio parahaemolyticus in oyster tissues after chlorine dioxide treatment. , 2010, International journal of food microbiology.

[10]  B. Mahmoud,et al.  Inactivation of Vibrio parahaemolyticus in pure culture, whole live and half shell oysters (Crassostrea virginica) by X‐ray , 2009, Letters in applied microbiology.

[11]  Yi-Cheng Su,et al.  Effects of flash freezing, followed by frozen storage, on reducing Vibrio parahaemolyticus in Pacific raw oysters (Crassostrea gigas). , 2009, Journal of food protection.

[12]  K. Morin.  Foodborne infections. , 2009, MCN. The American journal of maternal child nursing.

[13]  Yun-Ji Kim,et al.  Occurrence of Vibrio parahaemolyticus in oysters from Korean retail outlets , 2008 .

[14]  Haiqiang Chen,et al.  Conditions for high pressure inactivation of Vibrio parahaemolyticus in oysters. , 2008, International journal of food microbiology.

[15]  Haiqiang Chen,et al.  Conditions for a 5-log reduction of Vibrio vulnificus in oysters through high hydrostatic pressure treatment. , 2008, International journal of food microbiology.

[16]  L. Jaykus,et al.  An Overview of Vibrio vulnificus and Vibrio parahaemolyticus , 2007 .

[17]  Yi-Cheng Su,et al.  Vibrio parahaemolyticus: a concern of seafood safety. , 2007, Food microbiology.

[18]  J. Bandekar,et al.  RADIATION CONTROL OF VIBRIO PARAHAEMOLYTICUS IN SHRIMP , 2007 .

[19]  J. Kerry,et al.  Effects of high-pressure and heat treatments on physical and biochemical characteristics of oysters (Crassostrea gigas) , 2007 .

[20]  S. Fukuzaki.  Mechanisms of actions of sodium hypochlorite in cleaning and disinfection processes. , 2006, Biocontrol science.

[21]  Arun Sharma,et al.  Safe and shelf-stable natural casing using hurdle technology , 2006 .

[22]  A. Demirci,et al.  Electrolyzed oxidizing water treatment for decontamination of raw salmon inoculated with Escherichia coli O157:H7 and Listeria monocytogenes Scott A and response surface modeling , 2006 .

[23]  P. Mañas,et al.  Microbial inactivation by new technologies of food preservation , 2005, Journal of applied microbiology.

[24]  H. Heinonen‐Tanski,et al.  Inactivation of enteric microorganisms with chemical disinfectants, UV irradiation and combined chemical/UV treatments. , 2005, Water research.

[25]  Lee Chang Hee,et al.  Isolation of Major foodborne Pathogenic Bacteria from Ready-to-Eat Seafoods and Its Reduction Strategy , 2005 .

[26]  E. Russek-Cohen,et al.  Evaluation of chlorines' impact on biofilms on scratched stainless steel surfaces. , 2004, Bioresource technology.

[27]  Xuetong Fan,et al.  Effect of citric acid on the radiation resistance of Listeria monocytogenes and frankfurter quality factors. , 2003, Meat science.

[28]  K. Mallikarjunan,et al.  Low Dose Gamma Irradiation to Reduce Pathogenic Vibrios in Live Oysters (Crassostrea virginica) , 2003 .

[29]  Jui-Sen Peng,et al.  Inactivation and removal of Bacillus cereus by sanitizer and detergent. , 2002, International journal of food microbiology.

[30]  A. DePaola,et al.  Growth and survival of Vibrio parahaemolyticus in postharvest American oysters. , 2002, Journal of food protection.

[31]  E. Suffredini,et al.  Effects of depuration of molluscs experimentally contaminated with Escherichia coli, Vibrio cholerae O1 and Vibrio parahaemolyticus , 2002, Journal of applied microbiology.

[32]  M. Bhide,et al.  Presensitization of microorganisms by acid treatments to low dose gamma irradiation with special reference to Bacillus cereus. , 2001, Meat science.

[33]  C. Kaysner,et al.  Evaluation of Two Direct Plating Methods Using Nonradioactive Probes for Enumeration of Vibrio parahaemolyticus in Oysters , 2001, Applied and Environmental Microbiology.

[34]  C. Pollack,et al.  Outbreak of Vibrio parahaemolyticus infection associated with eating raw oysters and clams harvested from Long Island Sound--Connecticut, New Jersey, and New York, 1998. , 1999, MMWR. Morbidity and mortality weekly report.

[35]  J. Henkel.  Irradiation: a safe measure for safer food. , 1998, FDA consumer.

[36]  D. W. Cook,et al.  Cold Storage and Mild Heat Treatment as Processing Aids to Reduce the Numbers of Vibrio vulnificus in Raw Oysters. , 1992, Journal of food protection.

[37]  J. D. Kaylor,et al.  POTENTIAL OF IRRADIATION TECHNOLOGY FOR IMPROVED SHELLFISH SANITATION , 1991 .

[38]  J. T. Peeler,et al.  Incidence of Vibrio parahaemolyticus in U.S. coastal waters and oysters , 1990, Applied and environmental microbiology.

[39]  Hilda Ratner.  Vibrio vulnificus , 1987, Infection Control.

[40]  R. Grodner,et al.  RADIATION PASTEURIZATION OF GULF COAST OYSTERS , 1966 .