Micropreparative high-performance liquid chromatography of proteins and peptides.

The use of short microbore reversed phase and ion-exchange HPLC columns in the preparation of low level (submicrogram) quantities of proteins and peptides is discussed. The sequential use of columns of differing selectivity to purify complex mixtures is described. An example is given of the use of microbore columns to purify a murine myeloid leukemia inhibitory factor prior to sequence analysis.

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