Effects of type β transforming growth factors on haematopoietic progenitor cells

The effects of type β transforming growth factors (TGF‐βs) on normal human and murine haematopoietic progenitor cells were examined using bone marrow colony assays. In erythroid colony assays, TGF‐β1 inhibited human CFU‐E derived colony formation, BFU‐E derived burst formation, and murine BFU‐E derived burst formation in a dose dependent manner between 0.1 and 5.0 ng/ml. However, murine CFU‐E derived colony formation was unaffected even at a concentration of 5.0 ng/ml TGF‐β1. In myeloid colony assays, different sensitivity of progenitor cells to the inhibitory effects of TGF‐βs was observed between both species. TGF‐β1 inhibited murine granulocyte‐macrophage colony (GM‐colony) formation and granulocyte colony (G‐colony) formation in a dose dependent manner between 0.1 and 5.0 ng/ml, but had no remarkable effects on human GM‐colony and G‐colony formation. TGF‐β2 also had similar inhibitory effects on haematopoietic progenitor cells, while its inhibitory effect was less potent than that of TGF‐β1. Thus our data suggest that TGF‐β may be involved in negative regulation of haematopoiesis and that its inhibitory action may be restricted in lineage and/or species specific manner.

[1]  B. Haendler,et al.  Complementary DNA for human glioblastoma‐derived T cell suppressor factor, a novel member of the transforming growth factor‐beta gene family. , 1987, The EMBO journal.

[2]  A. Bassols,et al.  Two forms of transforming growth factor-β distinguished by multipotential haematopoietic progenitor cells , 1987, Nature.

[3]  H. Marquardt,et al.  Complete amino acid sequence of human transforming growth factor type beta 2. , 1987, The Journal of biological chemistry.

[4]  M. Sporn,et al.  Expression and secretion of type beta transforming growth factor by activated human macrophages. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[5]  L M Wakefield,et al.  Transforming growth factor type beta induces monocyte chemotaxis and growth factor production. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[6]  K. Frei,et al.  T cell suppressor factor from human glioblastoma cells is a 12.5‐kd protein closely related to transforming growth factor‐beta. , 1987, The EMBO journal.

[7]  S. Burstein,et al.  Type beta transforming growth factor is a potent inhibitor of murine megakaryocytopoiesis in vitro. , 1987, Blood.

[8]  Gary R. Grotendorst,et al.  TGF-β inhibition of endothelial cell proliferation: Alteration of EGF binding and EGF-induced growth-regulatory (competence) gene expression , 1987, Cell.

[9]  J. Massagué,et al.  The transforming growth factor-β system, a complex pattern of cross-reactive ligands and receptors , 1987, Cell.

[10]  C. Begley,et al.  Purification and properties of bacterially synthesized human granulocyte-macrophage colony stimulating factor. , 1987, Blood.

[11]  M. Sporn,et al.  Transforming growth factor beta is an important immunomodulatory protein for human B lymphocytes. , 1986, Journal of immunology.

[12]  D. Rosen,et al.  Antibodies to the N-terminal portion of cartilage-inducing factor A and transforming growth factor beta. Immunohistochemical localization and association with differentiating cells. , 1986, The Journal of biological chemistry.

[13]  M. Sporn,et al.  Transforming growth factor-beta: biological function and chemical structure. , 1986, Science.

[14]  K. Arai,et al.  Expression of murine and human granulocyte‐macrophage colony‐stimulating factors in S. cerevisiae: mutagenesis of the potential glycosylation sites. , 1986, The EMBO journal.

[15]  M. Sporn,et al.  Effects of transforming growth factor beta on the functions of natural killer cells: depressed cytolytic activity and blunting of interferon responsiveness. , 1986, Journal of immunology.

[16]  M. Sporn,et al.  Production of transforming growth factor beta by human T lymphocytes and its potential role in the regulation of T cell growth , 1986, The Journal of experimental medicine.

[17]  E. Chen,et al.  The murine transforming growth factor-beta precursor. , 1986, The Journal of biological chemistry.

[18]  J. Gabrilove,et al.  Recombinant human granulocyte colony-stimulating factor: effects on normal and leukemic myeloid cells. , 1986, Science.

[19]  C. H. Lin,et al.  Cloning and expression of the human erythropoietin gene. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[20]  Anita B. Roberts,et al.  Human transforming growth factor-β complementary DNA sequence and expression in normal and transformed cells , 1985, Nature.

[21]  K. Ozawa,et al.  Recombinant immune interferon inhibits leukemic cell growth by a monocyte-macrophage-mediated mechanism. , 1985, Experimental hematology.

[22]  M. Sporn,et al.  Type beta transforming growth factor: a bifunctional regulator of cellular growth. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[23]  M. Sporn,et al.  Transforming growth factor-beta in human platelets. Identification of a major storage site, purification, and characterization. , 1983, The Journal of biological chemistry.

[24]  M. Sporn,et al.  Transforming growth factors from neoplastic and nonneoplastic tissues. , 1983, Federation proceedings.

[25]  M. Sporn,et al.  New class of transforming growth factors potentiated by epidermal growth factor: isolation from non-neoplastic tissues. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[26]  T. Suda,et al.  A new technique for the cytochemical examination of human hemopoietic cells grown in agar gel. , 1980, Experimental hematology.