Regenerative NanoOctopus Based on Multivalent-Aptamer-Functionalized Magnetic Microparticles for Effective Cell Capture in Whole Blood.

Isolation of specific rare cell subtypes from whole blood is critical in cellular analysis and important in basic and clinical research. Traditional immunomagnetic cell capture suffers from suboptimal sensitivity, specificity, and time- and cost-effectiveness. Mimicking the features of octopuses, a device termed a "NanoOctopus" was developed for cancer cell isolation in whole blood. The device consists of long multimerized aptamer DNA strands, or tentacle DNA, immobilized on magnetic microparticle surfaces. Their ultrahigh sensitivity and specificity are attributed to multivalent binding of the tentacle DNA to cell receptors without steric hindrance. The simple, quick, and noninvasive capture and release of the target cells allows for extensive downstream cellular and molecular analysis, and the time- and cost-effectiveness of fabrication and regeneration of the devices makes them attractive for industrial manufacture.

[1]  Priya Balasubramanian,et al.  Antibody-independent capture of circulating tumor cells of non-epithelial origin with the ApoStream® system , 2017, PloS one.

[2]  Periodic assembly of nanospecies on repetitive DNA sequences generated on gold nanoparticles by rolling circle amplification. , 2008, Methods in molecular biology.

[3]  Hao Chen,et al.  Molecular Profiling of Pooled Circulating Tumor Cells from Prostate Cancer Patients Using a Dual-Antibody-Functionalized Microfluidic Device. , 2018, Analytical chemistry.

[4]  Xiaochun Xu,et al.  Specific Capture and Release of Circulating Tumor Cells Using Aptamer‐Modified Nanosubstrates , 2013, Advanced materials.

[5]  Lei Jiang,et al.  Antibody‐Modified Reduced Graphene Oxide Films with Extreme Sensitivity to Circulating Tumor Cells , 2015, Advanced materials.

[6]  M. Ali,et al.  Rolling circle amplification: applications in nanotechnology and biodetection with functional nucleic acids. , 2008, Angewandte Chemie.

[7]  Sven Frokjaer,et al.  Particle size and surface charge affect particle uptake by human dendritic cells in an in vitro model. , 2005, International journal of pharmaceutics.

[8]  J. Lis,et al.  A systematic study of the features critical for designing a high avidity multivalent aptamer. , 2013, Nucleic acid therapeutics.

[9]  X. Le,et al.  Rolling circle amplification: a versatile tool for chemical biology, materials science and medicine. , 2014, Chemical Society reviews.

[10]  Alexander van Reenen,et al.  Accelerated particle-based target capture--the roles of volume transport and near-surface alignment. , 2013, The journal of physical chemistry. B.

[11]  Jongyoon Han,et al.  Ultra-fast, label-free isolation of circulating tumor cells from blood using spiral microfluidics , 2015, Nature Protocols.

[12]  Shutao Wang,et al.  Frosted Slides Decorated with Silica Nanowires for Detecting Circulating Tumor Cells from Prostate Cancer Patients. , 2018, ACS applied materials & interfaces.

[13]  A. Fire,et al.  Rolling replication of short DNA circles. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[14]  F. Puppo,et al.  The cell polarity PTK7 receptor acts as a modulator of the chemotherapeutic response in acute myeloid leukemia and impairs clinical outcome. , 2010, Blood.

[15]  D. Shangguan,et al.  Aptamers evolved from live cells as effective molecular probes for cancer study , 2006, Proceedings of the National Academy of Sciences.

[16]  Hui Chen,et al.  Molecular recognition of acute myeloid leukemia using aptamers , 2008, Leukemia.

[17]  M. Toner,et al.  Enhanced Isolation and Release of Circulating Tumor Cells Using Nanoparticle Binding and Ligand Exchange in a Microfluidic Chip. , 2017, Journal of the American Chemical Society.

[18]  Z. Liao,et al.  Cellular Organelle-Dependent Cytotoxicity of Iron Oxide Nanoparticles and Its Implications for Cancer Diagnosis and Treatment: A Mechanistic Investigation , 2016 .

[19]  N. Palanisamy,et al.  Tunable Thermal‐Sensitive Polymer–Graphene Oxide Composite for Efficient Capture and Release of Viable Circulating Tumor Cells , 2016, Advances in Materials.

[20]  Vilmundur Gudnason,et al.  Heterogeneity in White Blood Cells Has Potential to Confound DNA Methylation Measurements , 2012, PloS one.

[21]  Seungpyo Hong,et al.  Dendrimer-mediated multivalent binding for the enhanced capture of tumor cells. , 2011, Angewandte Chemie.

[22]  Yuanjin Zhao,et al.  Aptamer‐Functionalized Barcode Particles for the Capture and Detection of Multiple Types of Circulating Tumor Cells , 2014, Advanced materials.

[23]  D. Truhlar.  Nearly Encounter-Controlled Reactions: The Equivalence of the Steady-State and Diffusional Viewpoints. , 1985 .

[24]  S. Gambhir,et al.  An intravascular magnetic wire for the high-throughput retrieval of circulating tumour cells in vivo , 2018, Nature Biomedical Engineering.

[25]  Zhi Zhu,et al.  Isolation, Detection, and Antigen-Based Profiling of Circulating Tumor Cells Using a Size-Dictated Immunocapture Chip. , 2017, Angewandte Chemie.

[26]  Mariya Vorobyeva,et al.  Multivalent Aptamers: Versatile Tools for Diagnostic and Therapeutic Applications , 2016, Molecules.

[27]  J. Gooding,et al.  Locked nucleic acid molecular beacon for multiplex detection of loop mediated isothermal amplification , 2018, Sensors and Actuators B: Chemical.

[28]  Ru-Fang Yeh,et al.  Molecular Biomarker Analyses Using Circulating Tumor Cells , 2010, PloS one.

[29]  Khalid K. Alam,et al.  Modular cell-internalizing aptamer nanostructure enables targeted delivery of large functional RNAs in cancer cell lines , 2018, Nature Communications.

[30]  Chunhai Fan,et al.  Simultaneous isolation and detection of circulating tumor cells with a microfluidic silicon-nanowire-array integrated with magnetic upconversion nanoprobes. , 2015, Biomaterials.

[31]  D. Campana,et al.  Use of peripheral blood instead of bone marrow to monitor residual disease in children with acute lymphoblastic leukemia. , 2002, Blood.

[32]  Jingying Li,et al.  Cell-Capture and Release Platform Based on Peptide-Aptamer-Modified Nanowires. , 2016, ACS applied materials & interfaces.

[33]  Weihua Huang,et al.  High-Efficiency Capture of Individual and Cluster of Circulating Tumor Cells by a Microchip Embedded with Three-Dimensional Poly(dimethylsiloxane) Scaffold. , 2016, Analytical chemistry.

[34]  Si-Yang Zheng,et al.  Size‐based separation methods of circulating tumor cells , 2018, Advanced drug delivery reviews.

[35]  Weian Zhao,et al.  Bioinspired multivalent DNA network for capture and release of cells , 2012, Proceedings of the National Academy of Sciences.

[36]  Weihong Tan,et al.  Multivalent DNA nanospheres for enhanced capture of cancer cells in microfluidic devices. , 2013, ACS nano.

[37]  J. Troge,et al.  Tumour evolution inferred by single-cell sequencing , 2011, Nature.

[38]  Dinshaw J. Patel,et al.  A multivalent DNA aptamer specific for the B-cell receptor on human lymphoma and leukemia , 2010, Nucleic acids research.

[39]  A. Bardelli,et al.  Integrating liquid biopsies into the management of cancer , 2017, Nature Reviews Clinical Oncology.