Experiments and numerical simulations under stationary and rotating conditions have been conducted to investigate turbulent flow and heat transfer characteristics of innovative guiding pin fin arrays in a wedge-shaped channel, which models the internal cooling passages for gas turbine blade trailing edge. The Reynolds number range is 10,000-80,000, and the inlet rotation number range is 0-0.46. With the increase of Reynolds numbers, the enhancement of heat transfer performance with guiding pin fin arrays is significantly higher than that with conventional circular pin fin arrays. At the highest Reynolds number of Re=80,000, the overall Nusselt number of the channel with guiding pin fin arrays is about 33.7% higher than that of the channel with circular pin fin arrays under the stationary condition, and is about 23.0% higher than the latter under the rotating conditions. At the highest inlet rotation number of Ro=0.46, the heat transfer difference between the trailing side and leading side of the channel is significantly lower with the guiding pin fin arrays. Both the experiments and numerical simulations indicate that the heat transfer uniformity and enhancement of the channel endwall is significantly improved by the guiding pin fin arrays under stationary and rotating conditions, which provide more reasonable flow distribution in the wedge-shaped channel, and can further produce obviously improved heat transfer in the tip region for the trailing edge internal cooling channel.