Sensitive Detection of Escherichia coli O157:H7 in Food Products by Impedimetric Immunosensors

In this work, the development of an impedimetric label-free immunosensor for the detection of Escherichia coli O157:H7 is reported. Different immobilization techniques of monoclonal anti-E. coli were tested, in order to reach the very low limit of detections. The comparison between the immobilization procedures underlined the advantages of the oriented procedure and the use of a dendrimer, which allowed for immobilizing a higher number of antibody units, reaching a very high sensitivity. However, the use of activated ferrocene as electron-transferring mediator, which improved the electrical properties of the system, resulted in a very low limit of detection equal to 3 cfu/mL. This immunosensor was used to analyze milk and meat samples obtaining a good agreement with the results of the ELISA methods.

[1]  Y. Ying,et al.  Label-free capacitive immunosensor based on quartz crystal Au electrode for rapid and sensitive detection of Escherichia coli O157:H7. , 2011, Analytica chimica acta.

[2]  R. Pilloton,et al.  A New Label-Free Impedimetric Affinity Sensor Based on Cholinesterases for Detection of Organophosphorous and Carbamic Pesticides in Food Samples: Impedimetric Versus Amperometric Detection , 2017, Food and Bioprocess Technology.

[3]  María Pedrero,et al.  Gold screen-printed-based impedimetric immunobiosensors for direct and sensitive Escherichia coli quantisation. , 2009, Biosensors & bioelectronics.

[4]  R. Pilloton,et al.  A highly sensitive impedimetric label free immunosensor for Ochratoxin measurement in cocoa beans. , 2016, Food chemistry.

[5]  M. Pereira da Cunha,et al.  Detection of Escherichia coli O157:H7 with langasite pure shear horizontal surface acoustic wave sensors. , 2006, Biosensors & bioelectronics.

[6]  J Samitier,et al.  Highly sensitive detection of pathogen Escherichia coli O157:H7 by electrochemical impedance spectroscopy. , 2013, Biosensors & bioelectronics.

[7]  R. Pilloton,et al.  A new label-free impedimetric aptasensor for gluten detection , 2017 .

[8]  H. S. Kim,et al.  Functionalization of a poly(amidoamine) dendrimer with ferrocenyls and its application to the construction of a reagentless enzyme electrode. , 2000, Analytical chemistry.

[9]  Zirong Wu,et al.  Self-assembled monolayers-based immunosensor for detection of Escherichia coli using electrochemical impedance spectroscopy , 2008 .

[10]  Q. Gao,et al.  Electrogenerated chemiluminescence biosensor incorporating ruthenium complex-labelled Concanavalin A as a probe for the detection of Escherichia coli. , 2012, Biosensors & bioelectronics.

[11]  T. Heyduk,et al.  Fluorescent homogeneous immunosensors for detecting pathogenic bacteria. , 2010, Analytical biochemistry.

[12]  Kyuwon Kim,et al.  Enzyme-amplified electrochemical detection of DNA using electrocatalysis of ferrocenyl-tethered dendrimer. , 2003, Analytical chemistry.

[13]  Jiadong Huang,et al.  Enzymatic repairing amplification-based versatile signal-on fluorescence sensing platform for detecting pathogenic bacteria , 2017 .

[14]  Michael Thompson,et al.  Detection of infectious and toxigenic bacteria. , 2002, The Analyst.

[15]  Joseph Irudayaraj,et al.  A mixed self-assembled monolayer-based surface plasmon immunosensor for detection of E. coli O157:H7. , 2006, Biosensors & bioelectronics.

[16]  A. De,et al.  Label free polyaniline based impedimetric biosensor for detection of E. coli O157:H7 Bacteria , 2012 .

[17]  R. Pilloton,et al.  Impedimetric Label-Free Immunosensor on Disposable Modified Screen-Printed Electrodes for Ochratoxin A , 2016, Biosensors.

[18]  Evangelyn C. Alocilja,et al.  Fabrication of a disposable biosensor for Escherichia Coli O157:H7 detection , 2003 .

[19]  Ciara K O'Sullivan,et al.  Reagentless, reusable, ultrasensitive electrochemical molecular beacon aptasensor. , 2006, Journal of the American Chemical Society.

[20]  Qiao-juan Gong,et al.  Detection of Escherichia coli with a label-free impedimetric biosensor based on lectin functionalized mixed self-assembled monolayer , 2016 .

[21]  R. Buchanan,et al.  Foodborne Disease Significance of Escherichia coli O157:H7 and Other Enterohemorrhagic E. coli , 1997 .