Preparation of Cultured Cells Using High-Pressure Freezing and Freeze Substitution for Subsequent 2D or 3D Visualization in the Transmission Electron Microscope

[1]  K. Mcdonald,et al.  Out with the old and in with the new: rapid specimen preparation procedures for electron microscopy of sectioned biological material , 2014, Protoplasma.

[2]  P. Verkade,et al.  Infectious Bronchitis Virus Generates Spherules from Zippered Endoplasmic Reticulum Membranes , 2013, mBio.

[3]  K. McDonald,et al.  Freeze substitution in 3 hours or less , 2011, Journal of microscopy.

[4]  Matthias Chiquet,et al.  Electron microscopy of high pressure frozen samples: bridging the gap between cellular ultrastructure and atomic resolution , 2008, Histochemistry and Cell Biology.

[5]  C. Netherton,et al.  Rapid freeze‐substitution preserves membranes in high‐pressure frozen tissue culture cells , 2007, Journal of microscopy.

[6]  D. Studer,et al.  A new approach for cryofixation by high‐pressure freezing , 2001, Journal of microscopy.

[7]  P. Moreau,et al.  Immunocytochemistry of Lipids: Chemical Fixatives have Dramatic Effects on the Preservation of Tissue Lipids , 1999, The Histochemical Journal.

[8]  D. Studer,et al.  Evidence for a distinct water-rich layer surrounding collagen fibrils in articular cartilage extracellular matrix. , 1996, Journal of Structural Biology.

[9]  K. Richter,et al.  Vitrification depth can be increased more than 10‐fold by high‐pressure freezing , 1993 .

[10]  K. Zierold Cryofixation methods for ion localization in cells by electron probe microanalysis: a review , 1991, Journal of microscopy.

[11]  D. Vanhecke,et al.  Close-to-native ultrastructural preservation by high pressure freezing. , 2008, Methods in cell biology.

[12]  D. Studer,et al.  High pressure freezing comes of age. , 1989, Scanning microscopy. Supplement.