Investigation on the Efficacy of a Killed Salmonella pullorum Vaccine

This study was carried out to determine the efficacy of a formalin-killed alum-precipitated Salmonella pullorum vaccine prepared by the Livestock and Poultry Vaccine Research and Production Centre (LPVRPC), BAU, Mymensingh, Bangladesh. Immunization with this vaccine induced serum antibody titers that peaked in 2-week following both primary and booster vaccination (P<0.05), and started to decline following 4-week of both vaccinations. Both primary and booster vaccination induced detectable antibody responses that were able to react with whole cells S. pullorum as determined by passive haemagglutination test (PHA). Both vaccinated chicken and mice showed maximal resistance following challenge with a virulent isolate of S. pullorum (P<0.01). In differential leukocyte counts in mice, a significant increase of lymphocytes was observed after primary immunization (P<0.01). Sera from vaccinated chickens conferred superior protection over naive chickens from lethal challenge with S. pullorum (P<0.01). Finally, this result indicates that S. pullorum vaccine is effective for controlling pullorum disease and antibody is crucial for protection. DOI: http://dx.doi.org/10.3329/mh.v1i1.13707 Microbes and Health Vol.1(1) June 2012 pp.14-18

[1]  Lipi Rani Basak.  Investigation on the Efficacy of Salmonella Bivalent Vaccine , 2013 .

[2]  M. Rahman,et al.  A Retrospective Analysis of Chicken Diseases Diagnosed at The BRAC Poultry Disease Diagnostic Centre of Gazipur , 2009 .

[3]  M. Rahman,et al.  Experimental Pathogenesis of Pullorum Disease with the Local Isolate of Salmonella enterica serovar. enterica subspecies Pullorum in Pullets in Bangladesh , 2009 .

[4]  Md. Mer Mosharraf Hossain,et al.  Avian Salmonella Infection: Isolation and Identification of Organisms and Histopathological Study , 2008 .

[5]  M. Kamruzzaman,et al.  Seroprevalence and pathological study of Salmonella infections in layer chickens and isolation and identification of causal agents , 2008 .

[6]  Katsuji Okuda,et al.  Multivalent DNA vaccine protects mice against pulmonary infection caused by Pseudomonas aeruginosa. , 2006, Vaccine.

[7]  V. Sharma,et al.  Protective efficacy of maternal antibodies induced by Salmonella toxoid (vaccine). , 2005, Indian journal of experimental biology.

[8]  H. Rahman,et al.  Development and evaluation of Salmonella Toxoid vaccine for poultry , 2004 .

[9]  M. Hugh-jones,et al.  Evaluation of biosecurity in broiler breeders. , 1987, Avian diseases.

[10]  M. Matsumoto,et al.  A bacterin against fowl cholera in turkeys: protective quality of various preparations originated from broth cultures. , 1977, Avian diseases.

[11]  T. A. Dorsey.  Studies on fowl cholera. II. The correlation between biochemic classification and the serologic and immunologic nature of avian Pasteurella multocida strains. , 1963, Avian diseases.

[12]  K. Heddleston,et al.  Studies on pasteurellosis. IV. Killed fowl cholera vaccine absorbed on aluminiumhydroxide. , 1960 .