Folylpoly-gamma-glutamate carboxypeptidase from pig jejunum. Molecular characterization and relation to glutamate carboxypeptidase II.

Jejunal folylpoly-gamma-glutamate carboxypeptidase hydrolyzes dietary folates prior to their intestinal absorption. The complete folylpoly-gamma-glutamate carboxypeptidase cDNA was isolated from a pig jejunal cDNA library using an amplified homologous probe incorporating primer sequences from prostate-specific membrane antigen, a protein capable of folate hydrolysis. The cDNA encodes a 751-amino acid polypeptide homologous to prostate-specific membrane antigen and rat brain N-acetylated alpha-linked acidic dipeptidase. PC3 transfectant membranes exhibited activities of folylpoly-gamma-carboxypeptidase and N-acetylated alpha-linked acidic dipeptidase, while immunoblots using monoclonal antibody to native folylpoly-gamma-glutamate carboxypeptidase identified a glycoprotein at 120 kDa and a polypeptide at 84 kDa. The kinetics of native folylpoly-gamma-carboxypeptidase were expressed in membranes of PC3 cells transfected with either pig folylpoly-gamma-carboxypeptidase or human prostate-specific membrane antigen. Folylpoly-gamma-carboxypeptidase transcripts were identified at 2.8 kilobase pairs in human and pig jejunum, human and rat brain, and human prostate cancer LNCaP cells. Thus, pig folylpoly-gamma-carboxypeptidase, rat N-acetylated alpha-linked acidic dipeptidase, and human prostate-specific membrane antigen appear to represent varied expressions of the same gene in different species and tissues. The discovery of the jejunal folylpoly-gamma-carboxypeptidase gene provides a framework for future studies on relationships among these proteins and on the molecular regulation of intestinal folate absorption.

[1]  J. Coyle,et al.  Molecular characterization of human brain N-acetylated alpha-linked acidic dipeptidase (NAALADase). , 1998, The Journal of pharmacology and experimental therapeutics.

[2]  J. Coyle,et al.  Isolation and expression of a rat brain cDNA encoding glutamate carboxypeptidase II. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[3]  P. Dawson,et al.  Cloning and Characterization of a Novel Peptidase from Rat and Human Ileum* , 1997, The Journal of Biological Chemistry.

[4]  J. Neale,et al.  Molecular Cloning of a Peptidase Against N‐Acetylaspartylglutamate from a Rat Hippocampal cDNA Library , 1997, Journal of neurochemistry.

[5]  N. Rawlings,et al.  Structure of membrane glutamate carboxypeptidase. , 1997, Biochimica et biophysica acta.

[6]  S. Rubin,et al.  Human intestinal folate transport: cloning, expression, and distribution of complementary RNA. , 1997, Gastroenterology.

[7]  C. Cordon-Cardo,et al.  Prostate-specific membrane antigen expression in normal and malignant human tissues. , 1997, Clinical cancer research : an official journal of the American Association for Cancer Research.

[8]  C. Halsted,et al.  Folate binding protein: molecular characterization and transcript distribution in pig liver, kidney and jejunum. , 1996, The Biochemical journal.

[9]  E. Schneider,et al.  Human gamma-glutamyl hydrolase: cloning and characterization of the enzyme expressed in vitro. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[10]  B. Suffoletto,et al.  Prostate-specific membrane antigen: a novel folate hydrolase in human prostatic carcinoma cells. , 1996, Clinical cancer research : an official journal of the American Association for Cancer Research.

[11]  S. Rubin,et al.  Intestinal folate transport: identification of a cDNA involved in folate transport and the functional expression and distribution of its mRNA. , 1996, Biochimica et biophysica acta.

[12]  B Chevrier,et al.  The structure of the Aeromonas proteolytica aminopeptidase complexed with a hydroxamate inhibitor. Involvement in catalysis of Glu151 and two zinc ions of the co-catalytic unit. , 1996, European journal of biochemistry.

[13]  G. Shoham,et al.  Aminopeptidase from Streptomyces griseus: primary structure and comparison with other zinc-containing aminopeptidases. , 1996, European journal of biochemistry.

[14]  J. Coyle,et al.  Prostate-specific membrane antigen is a hydrolase with substrate and pharmacologic characteristics of a neuropeptidase. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[15]  J. Isaacs,et al.  Localization and physical mapping of the prostate-specific membrane antigen (PSM) gene to human chromosome 11. , 1995, Genomics.

[16]  George L. Wright,et al.  Detection and characterization of the prostate‐specific membrane antigen (PSMA) in tissue extracts and body fluids , 1995, International journal of cancer.

[17]  K. Maclennan,et al.  Prostate-specific membrane antigen: evidence for the existence of a second related human gene. , 1995, British Journal of Cancer.

[18]  W. Fair,et al.  Alternatively spliced variants of prostate-specific membrane antigen RNA: ratio of expression as a potential measurement of progression. , 1995, Cancer research.

[19]  W. Fair,et al.  Expression of the prostate-specific membrane antigen. , 1994, Cancer research.

[20]  K. Cowan,et al.  A novel cDNA restores reduced folate carrier activity and methotrexate sensitivity to transport deficient cells. , 1994, The Journal of biological chemistry.

[21]  B. Vallee,et al.  Cocatalytic zinc motifs in enzyme catalysis. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[22]  W. Fair,et al.  Molecular cloning of a complementary DNA encoding a prostate-specific membrane antigen. , 1993, Cancer research.

[23]  D. Marguet,et al.  Dipeptidyl peptidase IV (CD 26) gene expression in enterocyte-like colon cancer cell lines HT-29 and Caco-2. Cloning of the complete human coding sequence and changes of dipeptidyl peptidase IV mRNA levels during cell differentiation. , 1992, The Journal of biological chemistry.

[24]  J. Coyle,et al.  Immunocytochemical localization of the N‐acetyl‐aspartyl‐glutamate (NAAG) hydrolyzing enzyme N‐acetylated α‐linked acidic dipeptidase (NAALADase) , 1992, The Journal of comparative neurology.

[25]  R. Lamb,et al.  Topology of eukaryotic type II membrane proteins: Importance of N-terminal positively charged residues flanking the hydrophobic domain , 1991, Cell.

[26]  E. Stokstad,et al.  Folic Acid Metabolism In Health And Disease , 1990 .

[27]  M. Geisow A practical guide to protein and peptide purification for microsequencing , 1990 .

[28]  M. V. Doyle,et al.  Quantitation of mRNA by the polymerase chain reaction. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[29]  P. Matsudaira A Practical guide to protein and peptide purification for microsequencing , 1989 .

[30]  J. Coyle,et al.  Hydrolysis of the brain dipeptide N-acetyl-L-aspartyl-L-glutamate. Identification and characterization of a novel N-acetylated alpha-linked acidic dipeptidase activity from rat brain. , 1987, The Journal of biological chemistry.

[31]  T. T. Wang,et al.  Intracellular pteroylpolyglutamate hydrolase from human jejunal mucosa. Isolation and characterization. , 1986, The Journal of biological chemistry.

[32]  T. T. Wang,et al.  Pteroylpolyglutamate hydrolase from human jejunal brush borders. Purification and characterization. , 1986, The Journal of biological chemistry.

[33]  L. Kühn,et al.  The human transferrin receptor gene: genomic organization, and the complete primary structure of the receptor deduced from a cDNA sequence , 1984, Cell.

[34]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[35]  R. Doolittle,et al.  A simple method for displaying the hydropathic character of a protein. , 1982, Journal of molecular biology.

[36]  W. Rutter,et al.  Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. , 1979, Biochemistry.

[37]  F. Sanger,et al.  DNA sequencing with chain-terminating inhibitors. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[38]  W. D. Benton,et al.  Screening lambdagt recombinant clones by hybridization to single plaques in situ. , 1977, Science.

[39]  A. van der Eb,et al.  A new technique for the assay of infectivity of human adenovirus 5 DNA. , 1973, Virology.

[40]  P. Leder,et al.  Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. , 1972, Proceedings of the National Academy of Sciences of the United States of America.

[41]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[42]  C. Baugh,et al.  Radioactive assay of folic acid polyglutamate conjugase(s). , 1970, Analytical biochemistry.