3-hydroxy-3-methylglutaryl coenzyme A reductase activity in human hair roots.

The activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase (1.1.1.34) was measured in isolated human hair roots. In growing scalp hair roots, the amount of enzyme activity per cell was surprisingly high and was of the same order of magnitude as has been reported for human liver cells and for human fibroblasts cultured in the absence of low density lipoproteins. Hair root enzyme activity showed no diurnal variation, was unaffected by cholestyramine feeding, and was similar in 14 normal subjects, one patient with abetalipoproteinemia, and one homozygote and three heterozygotes with familial hypercholesterolemia. The activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase in human hair roots appears to be unrelated to the level of low density lipoproteins in the plasma.

[1]  J. Wilson,et al.  Regulation of human hair growth by steroid hormones. II. Androstenedione metabolism in isolated hairs. , 1974, The Journal of clinical endocrinology and metabolism.

[2]  V. Rodwell,et al.  3-Hydroxy-3-methylglutaryl CoA reductase and mevalonate kinase of Neurospora crassa. , 1974, Journal of lipid research.

[3]  M. Brown,et al.  Homozygous familial hypercholesterolemia: specificity of the biochemical defect in cultured cells and feasibility of prenatal detection. , 1974, American journal of human genetics.

[4]  S. Shefer,et al.  Determination of hepatic 3-hydroxy-3-methylglutaryl CoA reductase activity in man. , 1974, Journal of lipid research.

[5]  M. Brown,et al.  Familial hypercholesterolemia: identification of a defect in the regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity associated with overproduction of cholesterol. , 1973, Proceedings of the National Academy of Sciences of the United States of America.

[6]  M. Brown,et al.  3-Hydroxy-3-methylglutaryl coenzyme A reductase. Solubilization and purification of a cold-sensitive microsomal enzyme. , 1973, The Journal of biological chemistry.

[7]  M. Brown,et al.  Regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity in human fibroblasts by lipoproteins. , 1973, Proceedings of the National Academy of Sciences of the United States of America.

[8]  S. Goldfarb,et al.  Stimulatory effect of dietary lipid and cholestyramine on hepatic HMG CoA reductase. , 1972, Journal of lipid research.

[9]  Marvin D. Siperstein,et al.  Regulation of cholesterol biosynthesis in normal and malignant tissues. , 1970 .

[10]  V. Rodwell,et al.  Diurnal variation and cholesterol regulation of hepatic HMG-CoA reductase activity. , 1969, Biochemical and biophysical research communications.

[11]  F. Lynen,et al.  Rhythmic changes of hydroxymethylglutaryl coenzyme a reductase activity in livers of fed and fasted rats , 1969, FEBS letters.

[12]  A. Kandutsch,et al.  Prevention of cyclic and triton-induced increases in hydroxymethylglutaryl coenzyme A reductase and sterol synthesis by puromycin. , 1969, The Journal of biological chemistry.

[13]  A. Kandutsch.  CHAPTER XXV – Sterol Metabolism in Skin and Epidermis1 , 1964 .

[14]  H. Pollard,et al.  The cell population of human liver. , 1961, The Journal of laboratory and clinical medicine.

[15]  E. V. Van Scott,et al.  The growing hair roots of the human scalp and morphologic changes therein following amethopterin therapy. , 1957, The Journal of investigative dermatology.

[16]  N. Nicolaides,et al.  The site of sterol and squalene synthesis in the human skin. , 1955, The Journal of investigative dermatology.