论文信息 - Facile preparation of a fluorescence-labeled plasmid

Facile preparation of a fluorescence-labeled plasmid

We developed a facile method for labeling plasmid DNA with a fluorescent probe (FITC). The obtained FITC-labeled plasmid kept luciferase activity, and had enough fluorescence intensity to monitor with flow cytometer and confocal laser scanning microscope.

Y. Okahata | Toshinori Sato | Tsuyoshi Ishii

[1] K. Matsuura,et al. Conjugation of plasmid DNAs with lactose via diazocoupling enhances resistance to restriction enzymes and acquires binding affinity to galactose-specific lectin. , 1999, Biochemical and biophysical research communications.

[2] K. Matsuura,et al. Synthesis of DNA-Carbohydrate Conjugate via Diazocoupling: A New Class of Modified DNA with Enhanced Stability and Lectin-Recognition Ability. , 1999 .

[3] M. Wilchek,et al. p-Diazobenzoyl-biocytin: a new biotinylating reagent for DNA. , 1988, Nucleic acids research.

[4] D. Scherman,et al. Coupling of nuclear localization signals to plasmid DNA and specific interaction of the conjugates with importin alpha. , 1999, Bioconjugate chemistry.

[5] M. Bassik,et al. DNA vector chemistry: The covalent attachment of signal peptides to plasmid DNA , 1998, Nature Biotechnology.

[6] M. Prince,et al. Gene transfer: A review of methods and applications , 1998, Pathology.

[7] Y. Okahata,et al. Characterization, cell uptake, and subcellular distribution of DNA complexes with lipoglutamides having tetraethylene glycol tails. , 1997, Journal of biomaterials science. Polymer edition.