A non-enzymatic sensor for hydrogen peroxide based on polyaniline, multiwalled carbon nanotubes and gold nanoparticles modified Au electrode.

We describe the construction of a polyaniline (PANI), multiwalled carbon nanotubes (MWCNTs) and gold nanoparticles (AuNPs) modified Au electrode for determination of hydrogen peroxide without using peroxidase (HRP). The AuNPs/MWCNT/PANI composite film deposited on Au electrode was characterized by Scanning Electron Microscopy (SEM) and electrochemical methods. Cyclic voltammetric (CV) studies of the electrode at different stages of construction demonstrated that the modified electrode had enhanced electrochemical oxidation of H(2)O(2), which offers a number of attractive features to develop amperometric sensors based on split of H(2)O(2). The amperometric response to H(2)O(2) showed a linear relationship in the range from 3.0 μM to 600.0 μM with a detection limit of 0.3 μM (S/N = 3) and with high sensitivity of 3.3 mA μM(-1). The sensor gave accurate and satisfactory results, when employed for determination of H(2)O(2) in milk and urine.

[1]  M. Pandey,et al.  Novel chitosan/gold-MPA nanocomposite for sequence-specific oligonucleotide detection , 2010 .

[2]  Joseph Owino,et al.  Electrochemical detection of glyphosate herbicide using horseradish peroxidase immobilized on sulfonated polymer matrix. , 2009, Bioelectrochemistry.

[3]  N. Vermeulen,et al.  Biomarkers of free radical damage applications in experimental animals and in humans. , 1999, Free radical biology & medicine.

[4]  Karl Cammann,et al.  Chrono amperometric determination of hydrogen peroxide in swimming pool water using an ultramicroelectrode array , 1998 .

[5]  Jun‐Jie Zhu,et al.  Fabrication of a novel nonenzymatic hydrogen peroxide sensor based on Se/Pt nanocomposites , 2010 .

[6]  M. Yorek.  The Role of Oxidative Stress in Diabetic Vascular and Neural Disease , 2003, Free radical research.

[7]  Nidhi Chauhan,et al.  An amperometric uric acid biosensor based on multiwalled carbon nanotube-gold nanoparticle composite. , 2011, Analytical biochemistry.

[8]  Junwei Di,et al.  Electrodeposition of gold nanoparticles on indium/tin oxide electrode for fabrication of a disposable hydrogen peroxide biosensor. , 2009, Talanta.

[9]  Junhua Huang,et al.  Determination of Hydrogen Peroxide in Rainwater by Using a Polyaniline Film and Platinum Particles Co-Modified Carbon Fiber Microelectrode , 1998 .

[10]  Chiyo Matsubara,et al.  Oxo[5, 10, 15, 20-tetra(4-pyridyl)porphyrinato]titanium(IV): an ultra-high sensitivity spectrophotometric reagent for hydrogen peroxide , 1992 .

[11]  J. Kan,et al.  Selective uricase biosensor based on polyaniline synthesized in ionic liquid , 2007 .

[12]  F. Pérez,et al.  An Improved Chemiluminescence Method for Hydrogen Peroxide Determination in Plant Tissues , 2005, Plant Growth Regulation.

[13]  C. Bala,et al.  Synergistic effect of mediator-carbon nanotube composites for dehydrogenases and peroxidases based biosensors. , 2009, Bioelectrochemistry.

[14]  A. Tiwari,et al.  Synthesis and characterization of highly crystalline polyaniline film promising for humid sensor , 2010 .

[15]  S. Bhadauria,et al.  Performance of nanopolyaniline-fungal enzyme based biosensor for water pollution , 2011 .

[16]  Q. Li,et al.  A novel non-enzymatic hydrogen peroxide sensor based on Mn-nitrilotriacetate acid (Mn-NTA) nanowires. , 2010, Talanta.

[17]  Guo-Li Shen,et al.  Nanosized flower-like ZnO synthesized by a simple hydrothermal method and applied as matrix for horseradish peroxidase immobilization for electro-biosensing. , 2005, Journal of inorganic biochemistry.

[18]  A. Tiwari,et al.  Synthesis and Characterization of Biopolymer-Based Electrical Conducting Graft Copolymers , 2008 .

[19]  Vandana Singh,et al.  Synthesis, characterization, and hoping transport properties of HCl doped conducting biopolymer-co-polyaniline zwitterion hybrids , 2008 .

[20]  N. Olson,et al.  Spectrophotometric Determination of Hydrogen Peroxide in Milk , 1967 .

[21]  R. Singh,et al.  Electrochemical DNA biosensor for the detection of sanguinarine in adulterated mustard oil , 2010 .

[22]  A. Saifer,et al.  The photometric microdetermination of blood glucose with glucose oxidase. , 1958, The Journal of laboratory and clinical medicine.

[23]  R. Kumar,et al.  Nanofibrous polyaniline thin film prepared by plasma‐induced polymerization technique for detection of NO2 gas , 2010 .

[24]  Premamoy Ghosh,et al.  Characterization of poly(vinyl pyrrolidone) modified polyaniline prepared in stable aqueous medium , 1999 .

[25]  Erkang Wang,et al.  Synthesis and electrochemical applications of gold nanoparticles. , 2007, Analytica chimica acta.

[26]  Vandana Singh,et al.  Synthesis and characterization of electrical conducting chitosan-graft-polyaniline , 2007 .

[27]  M. A. Alonso-Lomillo,et al.  Horseradish peroxidase covalent grafting onto screen-printed carbon electrodes for levetiracetam chronoamperometric determination. , 2009, Analytical biochemistry.

[28]  B. Halliwell.  Oxidative stress, nutrition and health. Experimental strategies for optimization of nutritional antioxidant intake in humans. , 1996, Free radical research.

[29]  A. Gopalan,et al.  Horseradish Peroxidase (HRP) Immobilized Poly(aniline-co-m-aminophenol) Film Electrodes–fabrication and Evaluation as Hydrogen Peroxide Sensor , 2007, Sensors (Basel, Switzerland).

[30]  Jianguo Deng,et al.  Carbon nanotube–polyaniline hybrid materials , 2002 .

[31]  Qin Xu,et al.  Porous Gold-Nanoparticle−CaCO3 Hybrid Material: Preparation, Characterization, and Application for Horseradish Peroxidase Assembly and Direct Electrochemistry , 2006 .

[32]  A. Tiwari.  Gum Arabic‐Graft‐Polyaniline: An Electrically Active Redox Biomaterial for Sensor Applications , 2007 .

[33]  G. Xu,et al.  Electrodeposition of Platinum Nanoparticles on Multi-Walled Carbon Nanotubes for Electrocatalytic Oxidation of Methanol , 2006 .

[34]  Jun‐Jie Zhu,et al.  Nonenzymatic Hydrogen Peroxide Sensor Based on Three-dimensional Ordered Macroporous Gold Film Modified Electrode , 2010 .

[35]  P. Southwell-keely,et al.  A reagentless amperometric biosensor for hydrogen peroxide determination based on asparagus tissue and ferrocene mediation , 1995 .

[36]  A. Kafi,et al.  Potential application of hemoglobin as an alternative to peroxidase in a phenol biosensor , 2008 .