To Pool, or Not to Pool?

IN this issue of Genetics, [Futschik and Schlotterer (2010)][1] present one of the first and the most systematic explorations of the relative merits of pooling vs. individual sequencing for several genetics applications. They argue that pooling individuals is often more effective both for SNP

[1]  J. M. Smith,et al.  The hitch-hiking effect of a favourable gene. , 1974, Genetical research.

[2]  G. A. Watterson On the number of segregating sites in genetical models without recombination. , 1975, Theoretical population biology.

[3]  B. Charlesworth,et al.  The effect of deleterious mutations on neutral molecular variation. , 1993, Genetics.

[4]  F J Ayala,et al.  Evidence for positive selection in the superoxide dismutase (Sod) region of Drosophila melanogaster. , 1994, Genetics.

[5]  L. Kruglyak,et al.  An analysis of strategies for discovery of single‐nucleotide polymorphisms , 2000, Genetic epidemiology.

[6]  Molly Przeworski,et al.  The signature of positive selection at randomly chosen loci. , 2002, Genetics.

[7]  M. Olivier A haplotype map of the human genome , 2003, Nature.

[8]  Kevin R. Thornton,et al.  Approximate Bayesian Inference Reveals Evidence for a Recent, Severe Bottleneck in a Netherlands Population of Drosophila melanogaster , 2006, Genetics.

[9]  Yun S. Song,et al.  The Hitchhiking Effect on Linkage Disequilibrium Between Linked Neutral Loci , 2006, Genetics.

[10]  John Maynard Smith,et al.  The hitch-hiking effect of a favourable gene. , 1974, Genetical research.

[11]  Pardis C Sabeti,et al.  Genome-wide detection and characterization of positive selection in human populations , 2007, Nature.

[12]  Kevin R. Thornton,et al.  An Approximate Bayesian Estimator Suggests Strong, Recurrent Selective Sweeps in Drosophila , 2008, PLoS genetics.

[13]  G. Coop,et al.  An approximate likelihood for genetic data under a model with recombination and population splitting. , 2009, Theoretical population biology.

[14]  Justin C. Fay,et al.  Quantification of rare allelic variants from pooled genomic DNA , 2009, Nature Methods.

[15]  Detlef Weigel,et al.  Deep sequencing to reveal new variants in pooled DNA samples , 2009, Human mutation.

[16]  Michael Lynch,et al.  Estimation of Allele Frequencies From High-Coverage Genome-Sequencing Projects , 2009, Genetics.

[17]  P. Visscher,et al.  Rapid inexpensive genome-wide association using pooled whole blood. , 2009, Genome research.

[18]  A. Futschik,et al.  The Next Generation of Molecular Markers From Massively Parallel Sequencing of Pooled DNA Samples , 2010, Genetics.

[19]  W. Stephan,et al.  Searching for Footprints of Positive Selection in Whole-Genome SNP Data From Nonequilibrium Populations , 2010, Genetics.