Properties of a Milk Clotting Protease Isolated from Fruits of Bromelia balansae Mez

Abstract Unripe fruit extracts of Bromelia balansae Mez Bromeliaceae), whose principal endopeptidase is balansain I (isolated for anion exchange chromatography: pI = 5.45, molecular weight = 23192), exhibit pH profile with a maximum activity around pH 9.0 and are inhibited only by cysteine peptidases inhibitors. The alanine and glutamine derivatives of N?carbobenzoxy Lamino acid pnitrophenyl esters were strongly preferred by the enzyme. Enzymatic hydrolysis of milk and soy proteins yield characteristic patterns at pH 9.0. The Nterminal sequence showed very high homology (85 90%) with other known Bromeliaceae endopeptidases.

[1]  F. Avilés,et al.  Purification of balansain I, an endopeptidase from unripe fruits of Bromelia balansae Mez (Bromeliaceae). , 2000, Journal of agricultural and food chemistry.

[2]  B. Maras,et al.  Purification and characterization of macrodontain I, a cysteine peptidase from unripe fruits of Pseudananas macrodontes (Morr.) harms (Bromeliaceae). , 2000, Protein expression and purification.

[3]  M. Schmid,et al.  A cysteine endopeptidase with a C-terminal KDEL motif isolated from castor bean endosperm is a marker enzyme for the ricinosome, a putative lytic compartment , 1998, Planta.

[4]  J. Wagner,et al.  Thermal behavior, solubility and structural properties of soy concentrate hydrolyzed by new plant proteases , 1998 .

[5]  Helmut. Uhlig,et al.  Industrial enzymes and their applications , 1998 .

[6]  Neil D. Rawlings,et al.  Handbook of proteolytic enzymes , 1998 .

[7]  T. Edmunds,et al.  Complete amino acid sequence of ananain and a comparison with stem bromelain and other plant cysteine proteases. , 1997, The Biochemical journal.

[8]  Thomas L. Madden,et al.  Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. , 1997, Nucleic acids research.

[9]  T. Tranbarger,et al.  Structure and expression of a developmentally regulated cDNA encoding a cysteine protease (pseudotzain) from Douglas fir. , 1996, Gene.

[10]  S. Arai,et al.  Isolation and characterization of two distinct cDNA clones encoding corn seed cysteine proteinases. , 1995, Biochimica et biophysica acta.

[11]  M J Leonard,et al.  Purification and characterization of multiple forms of the pineapple-stem-derived cysteine proteinases ananain and comosain. , 1994, The Biochemical journal.

[12]  C. Cordle.  Control of food allergies using protein hydrolysates , 1994 .

[13]  D. Buttle,et al.  [38] Pineapple cysteine endopeptidases , 1994 .

[14]  D. Buttle,et al.  Pineapple cysteine endopeptidases. , 1994, Methods in enzymology.

[15]  Z. Ye,et al.  Gene Expression Patterns Associated with in Vitro Tracheary Element Formation in Isolated Single Mesophyll Cells of Zinnia elegans , 1993, Plant physiology.

[16]  K. Shinozaki,et al.  Structure and expression of two genes that encode distinct drought-inducible cysteine proteinases in Arabidopsis thaliana. , 1993, Gene.

[17]  J. van Beeumen,et al.  Isolation and preliminary characterization of the cysteine-proteinases from the latex of Carica candamarcensis Hook. , 1993, Biological chemistry Hoppe-Seyler.

[18]  T. Minamikawa,et al.  Cysteine Endopeptidase from Vigna mungo: Gene Structure and Expression , 1992 .

[19]  S. Arai,et al.  Molecular cloning and gibberellin-induced expression of multiple cysteine proteinases of rice seeds (oryzains). , 1991, The Journal of biological chemistry.

[20]  N. Priolo,et al.  New purified plant proteinases for the food industry , 1991 .

[21]  D. Watson,et al.  The amino acid sequence of chymopapain from Carica papaya. , 1990, The Biochemical journal.

[22]  L. Pearl,et al.  Selective cleavage of glycyl bonds by papaya proteinase IV , 1990, FEBS letters.

[23]  N. Rawlings,et al.  Stem bromelain: Amino acid sequence and implications for weak binding of cystatin , 1989, FEBS letters.

[24]  A. Shutov,et al.  Degradation of storage proteins in germinating seeds , 1987 .

[25]  J. Adler-Nissen,et al.  Enzymic Hydrolysis of Food Proteins , 1986 .

[26]  Steve L Taylor.  Immunologic and Allergic Properties of Cows' Milk Proteins in Humans. , 1986, Journal of food protection.

[27]  V. Coghlan,et al.  Cloning and sequencing of papain-encoding cDNA. , 1986, Gene.

[28]  A. Kilara.  Enzyme-modified protein food ingredients. , 1985 .

[29]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[30]  R. Heinicke.  Complementary enzyme actions in the clotting of milk. , 1953, Science.