Prevalence of Anisakis Larvae in Different Fish Species in Southern Albania: Five-Year Monitoring (2016–2020)

Anisakidae are nematodes that commonly parasitize in the coelomic cavity and viscera of several fish species. They can be found in flesh, which is why they have an important economic and public health impact. The aim of the current work was to assess the presence and prevalence of Anisakis larvae in fish species caught in the coastal area of the Karaburun Peninsula in Vlora Bay (Albania). A total of 856 of wild teleosts and 219 specimens of farmed fish were collected over a 5-year period (from 2016 to 2020). The results showed that out of a total of 1075 analyzed samples, 361 (33.58%) were parasitized with L3 larvae. In particular, only Solea vulgaris returned negative results, while Sparus aurata, Dicentrarchus labrax, and Sardinella aurita showed the lowest prevalence (4.55%, 9.17%, and 10.53%, respectively) and mean abundance (0.84, 1.19, and 0.92, respectively). Conversely, Scomber japonicus and Scomber scombrus showed the highest prevalence (74.07% and 68.00%, respectively) and mean abundance (188.24 and 249.82, respectively). The data suggest that the coastal area of the Karaburun Peninsula (southern Albania) may be a high-risk area for zoonotic diseases, and the consumption of raw or undercooked fish caught in the Vlora district could result in the acquisition of human anisakiasis. For these reasons, it is necessary to improve the surveillance plan.

[1]  M. Santos,et al.  Anisakis notification in fish: An assessment of the cases reported in the European Union rapid alert system for food and feed (RASFF) database , 2021 .

[2]  P. Smooker,et al.  Anisakis Nematodes in Fish and Shellfish- from infection to allergies , 2019, International journal for parasitology. Parasites and wildlife.

[3]  G. Pierce,et al.  Human health, legislative and socioeconomic issues caused by the fish-borne zoonotic parasite Anisakis: Challenges in risk assessment , 2019, Trends in Food Science & Technology.

[4]  Laura Azzarito,et al.  Prevalence of anisakid parasites in fish collected from Apulia region (Italy) and quantification of nematode larvae in flesh. , 2019, International journal of food microbiology.

[5]  M. Trelis,et al.  Prevalence and Risk of Anisakid Larvae in Fresh Fish Frequently Consumed in Spain: An Overview , 2019, Fishes.

[6]  M. J. Perteguer,et al.  Epidemiological Scenario of Anisakidosis in Spain Based on Associated Hospitalizations: The Tip of the Iceberg , 2018, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[7]  M. Morales-Suárez-Varela,et al.  Epidemiology and management of foodborne nematodiasis in the European Union, systematic review 2000–2016 , 2018, Pathogens and global health.

[8]  D. Gibson,et al.  Detection of ascaridoid nematode parasites in the important marine food-fish Conger myriaster (Brevoort) (Anguilliformes: Congridae) from the Zhoushan Fishery, China , 2018, Parasites & Vectors.

[9]  M. Tejada,et al.  Pathogenic potential of Anisakis L3 after freezing in domestic freezers , 2018 .

[10]  C. S. Svanevik,et al.  Anisakis species composition and infection characteristics in Atlantic mackerel, Scomber scombrus , from major European fishing grounds — reflecting changing fish host distribution and migration pattern , 2017, Fisheries Research.

[11]  G. Nascetti,et al.  Anisakis pegreffii (Nematoda: Anisakidae) in European anchovy Engraulis encrasicolus from the Mediterranean Sea: Fishing ground as a predictor of parasite distribution , 2017, Fisheries Research.

[12]  Amitha,et al.  A review on role of fish in human nutrition with special emphasis to essential fatty acid , 2018 .

[13]  G. Nascetti,et al.  Molecular Epidemiology of Anisakis and Anisakiasis: An Ecological and Evolutionary Road Map. , 2018, Advances in parasitology.

[14]  G. Nascetti,et al.  Reviewing biodiversity and epidemiological aspects of anisakid nematodes from the North-east Atlantic Ocean , 2017, Journal of Helminthology.

[15]  G. Pierce,et al.  Assessing the risk of an emerging zoonosis of worldwide concern: anisakiasis , 2017, Scientific Reports.

[16]  J. Guitian,et al.  Qualitative risk assessment of introduction of anisakid larvae in Atlantic salmon (Salmo salar) farms and commercialization of products infected with viable nematodes , 2016 .

[17]  K. Buchmann,et al.  Effects of anisakid nematodes Anisakis simplex (s.l.), Pseudoterranova decipiens (s.l.) and Contracaecum osculatum (s.l.) on fish and consumer health ☆ , 2016 .

[18]  M. Trelis,et al.  Potential risk analysis of human anisakiasis through the consumption of mackerel, Scomber scombrus, sold at Spanish supermarkets , 2016 .

[19]  G. Nascetti,et al.  Larval migration of the zoonotic parasite Anisakis pegreffii (Nematoda: Anisakidae) in European anchovy, Engraulis encrasicolus: Implications to seafood safety , 2016 .

[20]  M. Lalle,et al.  Proficiency testing carried out by the European Union Reference Laboratory for Parasites , 2015, Accreditation and Quality Assurance.

[21]  M. Tejada,et al.  Antigenicity of Anisakis simplex s.s. L3 in parasitized fish after heating conditions used in the canning processing. , 2015, Journal of the science of food and agriculture.

[22]  K. Morsy,et al.  Hysterothylacium aduncum (Nematoda, Anisakidae) with a new host record from the common sole Solea solea (Soleidae) and its role as a biological indicator of pollution , 2015, Parasitology Research.

[23]  P. Merella,et al.  Molecular and epidemiological data on Anisakis spp. (Nematoda: Anisakidae) in commercial fish caught off northern Sardinia (western Mediterranean Sea). , 2014, Veterinary parasitology.

[24]  Matilda Brokaj.  The Impact of the Gastronomic Offer in Choosing Tourism Destination: The Case of Albania , 2014 .

[25]  G. Z. Pekmezci,et al.  Molecular identification of Anisakis species (Nematoda: Anisakidae) from marine fishes collected in Turkish waters. , 2014, Veterinary parasitology.

[26]  I. Mladineo,et al.  Ecology and Genetic Structure of Zoonotic Anisakis spp. from Adriatic Commercial Fish Species , 2013, Applied and Environmental Microbiology.

[27]  N. Nieuwenhuizen,et al.  Anisakis--a food-borne parasite that triggers allergic host defences. , 2013, International journal for parasitology.

[28]  G. Lee,et al.  A Case of Anisakiasis Invading the Stomach and the Colon at the Same Time after Eating Anchovies , 2013, Clinical endoscopy.

[29]  J. Martín‐Sánchez,et al.  Epidemiology and molecular identification of Anisakis pegreffii (Nematoda: Anisakidae) in the horse mackerel Trachurus trachurus from northern Morocco , 2013, Journal of Helminthology.

[30]  G. Nascetti,et al.  Anisakiasis and Gastroallergic Reactions Associated with Anisakis pegreffii Infection, Italy , 2013, Emerging infectious diseases.

[31]  Á. F. González,et al.  Optimization of the pepsin digestion method for anisakids inspection in the fishing industry. , 2013, Veterinary parasitology.

[32]  M. Lalle,et al.  Anisakidae infection in fish of the Aegean Sea. , 2012, Veterinary parasitology.

[33]  A. Tamvakis,et al.  Anisakis spp. burden in Trachurus trachurus , 2012, Helminthologia.

[34]  S. Klimpel,et al.  Anisakis simplex (s.s.) larvae in wild Alaska salmon: no indication of post-mortem migration from viscera into flesh. , 2011, Diseases of aquatic organisms.

[35]  S. Magnino,et al.  Risk assessment of parasites in fishery products , 2011 .

[36]  I. Mladineo,et al.  Do captive conditions favor shedding of parasites in the reared Atlantic bluefin tuna (Thunnus thynnus)? , 2011, Parasitology international.

[37]  D. Hamer,et al.  Anisakidosis: Perils of the deep. , 2010, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[38]  G. Arcangeli,et al.  Inactivation of Anisakis simplex larvae in raw fish using high hydrostatic pressure treatments , 2010 .

[39]  Jun Suzuki,et al.  Risk factors for human Anisakis infection and association between the geographic origins of Scomber japonicus and anisakid nematodes. , 2010, International journal of food microbiology.

[40]  P. Kania,et al.  Nematode infections of maricultured and wild fishes in Danish waters: A comparative study , 2009 .

[41]  G. Marty.  Anisakid larva in the viscera of a farmed Atlantic salmon (Salmo salar) , 2008 .

[42]  M. Kennedy,et al.  Anisakis simplex: from Obscure Infectious Worm to Inducer of Immune Hypersensitivity , 2008, Clinical Microbiology Reviews.

[43]  H. Karl.  Nematode larvae in fish on the German market: 20 years of consumer related research , 2008 .

[44]  S. Çelen,et al.  Helminth parasites of digestive tract of some teleost fish caught in the Dardanelles at Çanakkale, Turkey , 2007, Helminthologia.

[45]  M. Fioravanti,et al.  Anisakiasis in anchovies (Engraulis encrasicholus) and sardines (Sardina pilchardus) caught along the Adriatic coast , 2006 .

[46]  S. D’Amelio,et al.  The occurrence and infection dynamics of Anisakis larvae in the black-scabbard fish, Aphanopus carbo, chub mackerel, Scomber japonicus, and oceanic horse mackerel, Trachurus picturatus from Madeira, Portugal , 2003, Journal of Helminthology.

[47]  H. Cabral,et al.  The diet of blue whiting, hake, horse mackerel and mackerel off Portugal , 2002 .

[48]  I. Ansotegui,et al.  Anisakis simplex: dangerous--dead and alive? , 2002, Trends in parasitology.

[49]  P. Galli,et al.  Distribution of Anisakis simplex in fish caught in the Ligurian Sea , 2000, Parasitology Research.

[50]  Lajos Rózsa,et al.  QUANTIFYING PARASITES IN SAMPLES OF HOSTS , 2000, The Journal of parasitology.

[51]  K. Lafferty,et al.  Parasitology meets ecology on its own terms: Margolis et al. revisited. , 1997, The Journal of parasitology.

[52]  F. Moravec.  Parasitic Nematodes of Freshwater Fishes of Europe , 1995 .

[53]  S. Gaafar,et al.  Standardized nomenclature of animal parasitic diseases (SNOAPAD). , 1988, Veterinary parasitology.

[54]  JOHN W. Smith.  The abundance of Anisakis simplex L3 in the body-cavity and flesh of marine teleosts , 1984 .

[55]  R. Overstreet,et al.  Review of Hysterothylacium and Iheringascaris (both previously =Thynnascaris) (Nematoda: Anisakidae) from the northern Gulf of Mexico , 1980 .