Temperature Sensitivity and Pressure Resistance of Mushroom Polyphenoloxidase

Measurements of residual enzyme activity and FTIR-spectroscopy revealed that mushroom polyphenoloxidase is a thermosensitive enzyme readily inactivated by temperatures exceeding 50°C. The enzyme is, however, very pressure stable. At room temperature no enzyme inactivation occurred at pressures up to 6 kbar. FTIR-spectroscopy revealed that a modification in enzyme conformation occurred below 9 kbar. Kinetic studies showed that pressure and temperature did not always act synergistically with respect to enzyme inactivation. FTIR-spectroscopy showed that pressure- or temperature-induced changes were considerably different. Both temperature and pressure-temperature inactivation were only slightly influenced by pH (in the pH-range 5.5-7.5).

[1]  M. Hendrickx,et al.  >Kinetics for heat and pressure‐temperature inactivation of bacillus subtilis α‐amylase , 1996 .

[2]  P. Butz,et al.  Ultra-high pressure processing of onions: chemical and sensory changes. , 1994 .

[3]  Monica Anese,et al.  Effect of high pressure treatments on peroxidase and polyphenoloxidase activities , 1994 .

[4]  R. Dawley,et al.  4‐Hexylresorcinol, a Potent Inhibitor of Mushroom Tyrosinase , 1993 .

[5]  Dietrich Knorr,et al.  Effects of high-hydrostatic-pressure processes on food safety and quality , 1993 .

[6]  D. Hoover.  Pressure effects on biological systems , 1993 .

[7]  G. M. Sapers.  Browning of foods: control by sulfites, antioxidants, and other means , 1993 .

[8]  W. S. Otwell,et al.  Inhibition of enzymatic browning in foods and beverages. , 1992, Critical reviews in food science and nutrition.

[9]  R. Hayashi,et al.  Activation of Polyphenoloxidase in Pear Fruits by High Pressure Treatment , 1991 .

[10]  P. Wong,et al.  A New Internal Pressure Calibrant for High-Pressure Infrared Spectroscopy of Aqueous Systems , 1989 .

[11]  W. Flurkey,et al.  Tyrosinase Activities and Isoenzymes in Three Strains of Mushrooms , 1989 .

[12]  John R. Whitaker,et al.  Effect of ascorbic acid, sodium bisulfite, and thiol compounds on mushroom polyphenol oxidase , 1984 .

[13]  J. Whitaker,et al.  Relation between structure of polyphenol oxidase and prevention of browning. , 1984, Advances in experimental medicine and biology.

[14]  Chang Yong Lee,et al.  Polyphenoloxidase from DeChaunac grapes , 1983 .

[15]  A. Kilara,et al.  Control of Enzymatic Browning in Processed Mushrooms (Agaricus bisporus) , 1983 .

[16]  L. Vámos-Vigyázó,et al.  Polyphenol oxidases and peroxidases in fruits and vegetables , 1981 .

[17]  M. Galeazzi,et al.  Isolation, Purification and Physicochemical Characterization of Polyphenoloxidases (PPO) from a Dwarf Variety of Banana (Musa cavendishii, L) , 1981 .

[18]  E Morild,et al.  The theory of pressure effects on enzymes. , 1981, Advances in protein chemistry.

[19]  P. Thomas,et al.  Thin-layer isoelectric focusing of polyphenoloxidase of Sephadex and its detection by the print technique. , 1978, Analytical biochemistry.

[20]  D. Halim.  Polyphenol oxidase of D'Anjou pears (Pyrus communis L.) , 1978 .

[21]  M. W. Montgomery,et al.  POLYPHENOL OXIDASE OF ROYAL ANN CHERRIES: PURIFICATION AND CHARACTERIZATION , 1973 .

[22]  K. Lerch,et al.  Purification and characterization of a tyrosinase from Streptomyces glaucescens. , 1972, European journal of biochemistry.

[23]  H. Yang,et al.  IDENTIFICATION AND CHARACTERIZATION OF SOME OXIDIZING ENZYMES OF THE MC FARLIN CRANBERRY , 1971 .

[24]  C. L. Bedford,et al.  Multiple Forms of Phenoloxidase , 1967 .

[25]  E. Harel,et al.  Assay of catechol oxidase—a critical comparison of methods , 1966 .

[26]  K. Suzuki,et al.  STUDIES ON TAKA-AMYLASE A UNDER HIGH PRESSURE. 1. SOME KINETIC ASPECTS OF PRESSURE INACTIVATION OF TAKA-AMYLASE A. , 1964, Archives of biochemistry and biophysics.