Killing Activity of Micafungin against Aspergillus fumigatus Hyphae Assessed by Specific Fluorescent Staining for Cell Viability

ABSTRACT We studied the anti-Aspergillus activity of micafungin by using two fluorescent dyes to detect cell viability. Micafungin induced flattened hyphae, caused by the bursting of cells, which had lost their viability. Micafungin has killing activity against actively growing hyphae, even though it is not fungicidal against the whole burden of Aspergillus fumigatus.

[1]  D. Schmatz,et al.  The Antifungal Echinocandin Caspofungin Acetate Kills Growing Cells of Aspergillus fumigatus In Vitro , 2002, Antimicrobial Agents and Chemotherapy.

[2]  F. Ikeda,et al.  Antifungal mechanism of FK463 against Candida albicans and Aspergillus fumigatus. , 2002, The Journal of antibiotics.

[3]  A. Beauvais,et al.  Glucan Synthase Complex of Aspergillus fumigatus , 2001, Journal of bacteriology.

[4]  F. Ikeda,et al.  Efficacy of FK463, a New Lipopeptide Antifungal Agent, in Mouse Models of Disseminated Candidiasis and Aspergillosis , 2000, Antimicrobial Agents and Chemotherapy.

[5]  F. Ikeda,et al.  Efficacy of FK463, a New Lipopeptide Antifungal Agent, in Mouse Models of Pulmonary Aspergillosis , 2000, Antimicrobial Agents and Chemotherapy.

[6]  Koji Kawabata,et al.  In Vitro Activities of a New Lipopeptide Antifungal Agent, FK463, against a Variety of Clinically Important Fungi , 2000, Antimicrobial Agents and Chemotherapy.

[7]  R. Rennie,et al.  Assessment of the Effect of Amphotericin B on the Vitality of Candida albicans , 1999, Antimicrobial Agents and Chemotherapy.

[8]  M H Zwietering,et al.  Characterization of uptake and hydrolysis of fluorescein diacetate and carboxyfluorescein diacetate by intracellular esterases in Saccharomyces cerevisiae, which result in accumulation of fluorescent product , 1995, Applied and environmental microbiology.

[9]  D. Epps,et al.  Characterization of the steady-state and dynamic fluorescence properties of the potential-sensitive dye bis-(1,3-dibutylbarbituric acid)trimethine oxonol (Dibac4(3)) in model systems and cells. , 1994, Chemistry and physics of lipids.

[10]  B. Haarer,et al.  Fluorescence microscopy methods for yeast. , 1989, Methods in cell biology.

[11]  A. Trinci,et al.  Effects of Junlon and Hostacerin on the Electrokinetic Properties of Spores of Aspergillus niger, Phanerochaete chrysosporium and Geotrichum candidum , 1988 .

[12]  E. Bruck,et al.  National Committee for Clinical Laboratory Standards. , 1980, Pediatrics.

[13]  B. Chance,et al.  The behavior of oxonol dyes in phospholipid dispersions. , 1979, Biophysical journal.