Characterization of Agro-diversity by Seed Storage Protein Electrophoresis: Focus on Rice Germplasm from Uttarakhand Himalaya, India

Abstract The characteristics of 48 rice varieties from Uttarakhand Himalaya, India were detected by morphological and biochemical markers. The grains of the selected rice varieties varied in their morphological (grain length, grain width and grain weight) and biochemical characters (sodium dodecyl sulfate polyacrylamide gel electrophoresis, SDS-PAGE). Based on the presence of 70, 65, 60, 57, 37–39, 22–23, 13 and 10 kDa protein bands in the 48 rice varieties, seven types of profiles were identified. An unweighted pair group average method with arithmetic mean (UPGMA) dendrogram based on cluster analysis of genetic similarity of the protein bands showed two distinct groups with 1%–78% similarity coefficients. The presence of characteristic bands in selected varieties is a useful parameter for identification of rice germplasm.

[1]  Darshan S. Brar,et al.  Rice genetics IV , 2008 .

[2]  M. Blair,et al.  Inter-simple sequence repeat (ISSR) amplification for analysis of microsatellite motif frequency and fingerprinting in rice (Oryza sativa L.) , 1999, Theoretical and Applied Genetics.

[3]  D. Brar,et al.  Phylogenetic relationships among Oryza species revealed by AFLP markers , 1999, Theoretical and Applied Genetics.

[4]  S. Jain,et al.  A manual of ethnobotany , 1995 .

[5]  M. Blair,et al.  Fluorescent-labeled microsatellite panels useful for detecting allelic diversity in cultivated rice (Oryza sativa L.) , 2002, Theoretical and Applied Genetics.

[6]  K. Holsinger,et al.  A comparison of methods for assessing genetic variation in plant conservation biology. , 1991 .

[7]  B. O. Juliano,et al.  Characterization of poorly digested protein of cooked rice protein bodies , 1993 .

[8]  Samuel W. Cartinhour,et al.  Microsatellite markers in rice: abundance, diversity, and applications. , 2001 .

[9]  K. Holsinger,et al.  Genetics and conservation of rare plants. , 1991 .

[10]  T. Kumamaru,et al.  Screening of Rice Varieties for Endosperm Storage Proteins , 1990 .

[11]  Jin Wei-dong.  Genetic Diversity of Seed Storage Proteins in Different Ecotype Varieties of japonica Rice and Its Application , 2006 .

[12]  N. Sharopova,et al.  Characterisation of European oat germ plasm: allelic variation at complex avenin loci detected by acid polyacrylamide gel electrophoresis , 1998, Euphytica.

[13]  A. Singh,et al.  Phylogenetic relationships in the genus Arachis based on seed protein profiles , 2004, Euphytica.

[14]  Kunisuke Tanaka,et al.  Mutants for rice storage proteins , 1988, Theoretical and Applied Genetics.

[15]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[16]  B. O. Juliano,et al.  Studies on the extraction and composition of rice proteins. , 1966 .

[17]  H. Satoh,et al.  Genetic variation in storage protein and storage endosperm starch in local rice cultivars of Myanmar. , 2003 .

[18]  K. Eskridge,et al.  Comparison of phenotypic and molecular marker-based classifications of hard red winter wheat cultivars , 2005, Euphytica.

[19]  Kunisuke Tanaka,et al.  Purification of protein body-I of rice seed and its polypeptide composition , 1987 .

[20]  Amanda J. Garris,et al.  Genetic Structure and Diversity in Oryza sativa L. , 2005, Genetics.

[21]  R. Sammour.  USING ELECTROPHORETIC TECHNIQUES IN VARIETAL IDENTIFICATION, BIOSYSTEMATIC ANALYSIS, PHYLOGENETIC RELATIONS AND GENETIC RESOURCES MANAGEMENT , 1991 .

[22]  M. Iwamoto,et al.  Comparison of Rice (Oryza sativa L.) Seed Proteins from Several Varieties , 1984 .

[23]  S. Hayashida,et al.  The Relationship of the Feces Protein Particles to Rice Protein Bodies , 1975 .

[24]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.