Comparison of a Micromethod for Performance of the Serum Bactericidal Test with the Standard Tube Dilution Method

A micromethod for performance of the serum bactericidal test is described, and the results obtained with this method are compared with those obtained with the standard tube dilution macromethod. An agreement within ± 1 dilution was achieved in 23 of 25 (92%) determinations of the serum bactericidal titer. The micromethod used approximately one-third of the amount of pooled normal human serum and of the technician's time required for performance of the macromethod. The micromethod offers an accurate and economical alternative to the macromethod for the performance of the serum bactericidal test and is particularly useful with infants and children since it minimizes blood loss.

[1]  G. Mccracken,et al.  Oral antibiotic therapy for skeletal infections of children. II. Therapy of osteomyelitis and suppurative arthritis. , 1978, The Journal of pediatrics.

[2]  C. Stratton,et al.  Serum dilution test for bactericidal activity. I. Selection of a physiologic diluent. , 1977, The Journal of infectious diseases.

[3]  K. Vosti,et al.  Comparison of Broth and Human Serum as the Diluent in the Serum Bactericidal Test , 1975, Antimicrobial Agents and Chemotherapy.

[4]  K. Vosti,et al.  Variation in Performance of the Serum Bactericidal Test , 1974, Antimicrobial Agents and Chemotherapy.

[5]  J. Klastersky,et al.  Antibacterial activity in serum and urine as a therapeutic guide in bacterial infections. , 1974, The Journal of infectious diseases.

[6]  L. Chitwood,et al.  Tube dilution antimicrobial susceptibility testing: efficacy of a microtechnique applicable to diagnostic laboratories. , 1969, Applied microbiology.

[7]  E. Jawetz.  Combined antibiotic therapy in serious infections caused by drug-resistant staphylococci (1956-1957). , 1959, A.M.A. archives of internal medicine.

[8]  J. G. Schlghter,et al.  EFFECTIVE PENICILLIN THERAPY IN SUBACUTE BACTERIAL ENDOCARDITIS AND OTHER CHRONIC INFECTIONS , 1949, The American journal of the medical sciences.

[9]  J. Schlichter,et al.  A method of determining the effective therapeutic level in the treatment of subacute bacterial endocarditis with penicillin; a preliminary report. , 1947, American heart journal.