Data, Models, Thresholds

The scientific method involves developing models (typically referred to as hypotheses) and then collecting and analyzing data to see how well the model fits the data. This chapter discusses the source of data used in DNA analysis and introduces models and thresholds important to DNA interpretation.

[1]  G. Scollary,et al.  A statistical overview of standard (IUPAC and ACS) and new procedures for determining the limits of detection and quantification: Application to voltammetric and stripping techniques (Technical Report) , 1997 .

[2]  G. Box Robustness in the Strategy of Scientific Model Building. , 1979 .

[3]  Jo-Anne Bright,et al.  Examination of the variability in mixed DNA profile parameters for the Identifiler multiplex. , 2010, Forensic science international. Genetics.

[4]  Lloyd A. Currie,et al.  Detection and quantification limits: origins and historical overview , 1997 .

[5]  H. Kaiser Report for analytical chemists. II. Quantitation in elemental analysis , 1970 .

[6]  Peter Gill,et al.  High-throughput analysis using AmpFlSTR® Identifiler® with the Applied Biosystems 3500xl Genetic Analyser. , 2013, Forensic science international. Genetics.

[7]  Duncan Taylor,et al.  The interpretation of single source and mixed DNA profiles. , 2013, Forensic science international. Genetics.

[8]  Catherine M Cupples,et al.  STR Profiles from DNA Samples with “Undetected” or Low Quantifiler™ Results , 2009, Journal of forensic sciences.

[9]  Titia Sijen,et al.  Higher Capillary Electrophoresis Injection Settings as an Efficient Approach to Increase the Sensitivity of STR Typing , 2009, Journal of forensic sciences.

[10]  Catherine M Grgicak,et al.  Maximizing allele detection: Effects of analytical threshold and DNA levels on rates of allele and locus drop-out. , 2012, Forensic science international. Genetics.

[11]  Catherine M. Grgicak,et al.  Analytical Thresholds and Sensitivity: Establishing RFU Thresholds for Forensic DNA Analysis , , 2013, Journal of forensic sciences.

[12]  J. D. Winefordner,et al.  Limit of detection. A closer look at the IUPAC definition , 1983 .

[13]  Stephen T Sherry,et al.  A Mathematical Approach to the Analysis of Multiplex DNA Profiles , 2011, Bulletin of mathematical biology.

[14]  S Watson,et al.  Practical determination of the low template DNA threshold. , 2011, Forensic science international. Genetics.

[15]  Simon Ford,et al.  Systematic differences in electropherogram peak heights reported by different versions of the GeneScan software. , 2004, Journal of forensic sciences.

[16]  F. J. Holler,et al.  Principles of Instrumental Analysis , 1973 .

[17]  John Buckleton,et al.  A universal strategy to interpret DNA profiles that does not require a definition of low-copy-number. , 2010, Forensic science international. Genetics.

[18]  Keith Inman,et al.  Run‐Specific Limits of Detection and Quantitation for STR‐based DNA Testing , 2007, Journal of forensic sciences.