Development of HPLC method for determination of phenolic compounds on a core shell column by direct injection of wine samples

Phenolic compounds are frequently present in various natural products, and they can have different beneficial biological potentials. The most widely used method for determination of individual phenolic compounds is high-performance liquid chromatography (HPLC). In this paper, a method for simultaneous determination of 16 phenolic compounds (gallic acid, p-hydroxybenzoic acid, catechin, syringic acid, trans-cinnamic acid, hesperetin, naringenin, vanillic acid, benzoic acid, coumaric acid, resveratrol, chlorogenic acid, caffeic acid, rutin, quercetin, and kaempferol) on a core–shell column was developed. The separation method conducted on a standard ODS (250 mm) column was transferred to Poroshell column and optimized using non-ultra-high-performance liquid chromatography (UHPLC) apparatus. Phenolic compounds were separated fast and efficiently during 30-min analysis, and validation parameters were determined. The developed method was successfully applied on the analysis of phenolic content after direct injection of red wines from three different grape varieties.

[1]  M. Bordignon-Luiz,et al.  Rapid determination of flavonoids and phenolic acids in grape juices and wines by RP-HPLC/DAD: Method validation and characterization of commercial products of the new Brazilian varieties of grape. , 2017, Food chemistry.

[2]  A. Versari,et al.  Phenolic compounds and antioxidant activity of Macedonian red wines , 2015 .

[3]  M. Atanacković,et al.  Effect of Wine Production Techniques on Wine Resveratrol and Total Phenolics , 2015 .

[4]  Víctor González-Ruiz,et al.  Core-shell particles lead the way to renewing high-performance liquid chromatography , 2015 .

[5]  M. Savić,et al.  Development and validation of an RP-HPLC method for quantification of trans-resveratrol in the plant extracts , 2015 .

[6]  T. Edge,et al.  Core-shell particles: preparation, fundamentals and applications in high performance liquid chromatography. , 2014, Journal of chromatography. A.

[7]  V. Preedy.  Processing and Impact on Active Components in Food , 2014 .

[8]  M. Chicharro,et al.  Classification of Spanish white wines using their electrophoretic profiles obtained by capillary zone electrophoresis with amperometric detection , 2014, Electrophoresis.

[9]  M. L. Escudero-Gilete,et al.  Comparative study of the enological potential of different winemaking byproducts: implications in the antioxidant activity and color expression of red wine anthocyanins in a model solution. , 2014, Journal of Agricultural and Food Chemistry.

[10]  D. Antonacci,et al.  Antithrombotic activity of 12 table grape varieties. Relationship with polyphenolic profile. , 2013, Food chemistry.

[11]  A. Klassen,et al.  Fast Determination of Phenolic Compounds in Brazilian Wines from Vale do São Francisco Region by CE , 2013, Chromatographia.

[12]  D. Manns,et al.  A core-shell column approach to a comprehensive high-performance liquid chromatography phenolic analysis of Vitis vinifera L. and interspecific hybrid grape juices, wines, and other matrices following either solid phase extraction or direct injection. , 2012, Journal of chromatography. A.

[13]  M. Atanacković,et al.  Influence of winemaking techniques on the resveratrol content, total phenolic content and antioxidant potential of red wines , 2012 .

[14]  M. Bordignon-Luiz,et al.  Determination of some phenolic compounds in red wine by RP-HPLC: method development and validation. , 2011, Journal of Chromatographic Science.

[15]  G. Guiochon,et al.  Shell particles, trials, tribulations and triumphs. , 2011, Journal of chromatography. A.

[16]  I. Žuntar,et al.  Phenol content, antioxidant activity and metal composition of Croatian wines deriving from organically and conventionally grown grapes , 2011 .

[17]  T. Saleem,et al.  Red wine: A drink to your heart , 2010, Journal of cardiovascular disease research.

[18]  M. Atanacković,et al.  Determination of trans- and cis-resveratrol in Serbian commercial wines. , 2010, Journal of chromatographic science.

[19]  Hua-Bin Li,et al.  Biological Activities of Polyphenols from Grapes , 2010, International journal of molecular sciences.

[20]  B. Kiss,et al.  A Rapid Method for Determination of Resveratrol in Wines by HPLC-MS , 2009 .

[21]  Eunhye Kim,et al.  Phenolic compound concentration and antioxidant activities of edible and medicinal mushrooms from Korea. , 2008, Journal of agricultural and food chemistry.

[22]  Weidong Huang,et al.  Effects of grape variety, harvest date, fermentation vessel and wine ageing on flavonoid concentration in red wines , 2008 .

[23]  S. Gómez-Alonso,et al.  HPLC analysis of diverse grape and wine phenolics using direct injection and multidetection by DAD and fluorescence , 2007 .

[24]  O. Gürbüz,et al.  Determination of flavan-3-ols and trans-resveratrol in grapes and wine using HPLC with fluorescence detection , 2007 .

[25]  Weidong Huang,et al.  Determination of red wine flavonoids by HPLC and effect of aging , 2007 .

[26]  Silvia Pérez-Magariño,et al.  Polyphenols and colour variability of red wines made from grapes harvested at different ripeness grade , 2006 .

[27]  M. Bronze,et al.  Analysis of phenolic compounds in Muscatel wines produced in Portugal , 2006 .

[28]  Nelson Durán,et al.  Phenolic compounds and total antioxidant potential of commercial wines , 2003 .

[29]  F. Martínez-Martínez,et al.  The Study of Phenolic Compounds as Natural Antioxidants in Wine , 2003, Critical reviews in food science and nutrition.

[30]  J. Mérillon,et al.  Direct liquid chromatographic analysis of resveratrol derivatives and flavanonols in wines with absorbance and fluorescence detection , 2002 .

[31]  S. Haroutounian,et al.  Direct HPLC Assay of Five Biologically Interesting Phenolic Antioxidants in Varietal Greek Red Wines , 2001 .

[32]  D. Goldberg,et al.  Catechin and Epicatechin Concentrations of Red Wines: Regional and Cultivar-Related Differences , 1998, American Journal of Enology and Viticulture.

[33]  D. Goldberg,et al.  Toward the Fingerprinting of Wines: Cultivar-Related Patterns of Polyphenolic Constituents in Ontario Wines , 1997 .

[34]  Lloyd R. Snyder,et al.  Practical HPLC Method Development: Snyder/Practical , 1997 .