Photoacoustic calorimetry of proteins.

We have described two examples of time-resolved photoacoustic calorimetry for the study of heme protein transient intermediates. Before photoacoustic calorimetry, determining thermodynamic information on short-lived intermediates was difficult. Along with being sensitive to enthalpic and volume changes, photoacoustic calorimetry can detect conformational changes in a time-resolved manner. In complex protein systems, the interpretation of the structural origins of a conformational change is sometimes difficult. Site-directed mutagenesis has been used successfully to identify the residues that play important roles in the ligand binding to both Mb and cytochrome P450cam. In both systems the hydration state of salt bridges gave rise to volume changes that were identified through mutagenesis of the residues involved. With its increasing popularity and the power of site-directed mutagenesis, time-resolved photoacoustic calorimetry is fast becoming a technique to probe conformational dynamics in proteins.

[1]  K. Ishimori,et al.  High-pressure laser photolysis study of hemoproteins. Effects of pressure on carbon monoxide binding dynamics for R- and T-state hemoglobins. , 1990, Biochemistry.

[2]  J. Westrick,et al.  A time-resolved photoacoustic calorimetry study of the dynamics of enthalpy and volume changes produced in the photodissociation of carbon monoxide from sperm whale carboxymyoglobin. , 1987, Biochemistry.

[3]  I. C. Gunsalus,et al.  Bacterial P-450cam methylene monooxygenase components: cytochrome m, putidaredoxin, and putidaredoxin reductase. , 1978, Methods in enzymology.

[4]  A. V. Wells,et al.  Measurements of CO Geminate Recombination in Cytochromes P450 and P420 (*) , 1995, The Journal of Biological Chemistry.

[5]  M Karplus,et al.  X-ray structure and refinement of carbon-monoxy (Fe II)-myoglobin at 1.5 A resolution. , 1986, Journal of molecular biology.

[6]  B C Finzel,et al.  The 2.6-A crystal structure of Pseudomonas putida cytochrome P-450. , 1985, The Journal of biological chemistry.

[7]  C. Di Primo,et al.  Conformational dynamics of cytochrome P-450cam as monitored by photoacoustic calorimetry. , 1993, Biochemistry.

[8]  T. Logan,et al.  Photoacoustic calorimetry study of human carboxyhemoglobin , 1992 .

[9]  J. Olson,et al.  Ligand binding to heme proteins. An evaluation of distal effects. , 1983, The Journal of biological chemistry.

[10]  C. Di Primo,et al.  Origin of the photoacoustic signal in cytochrome P-450cam: role of the Arg186-Asp251-Lys178 bifurcated salt bridge. , 1997, Biochemistry.

[11]  R. Raag,et al.  Crystal structure of the carbon monoxide-substrate-cytochrome P-450CAM ternary complex. , 1989, Biochemistry.

[12]  A. V. Wells,et al.  Resonance Raman investigations of Escherichia coli-expressed Pseudomonas putida cytochrome P450 and P420. , 1992, Biochemistry.

[13]  D. E. Kerr,et al.  Reaction of myoglobin with phenylhydrazine: a molecular doorstop. , 1984, Biochemistry.

[14]  J. Goodman,et al.  Simultaneous determination of photoreaction dynamics and energetics using pulsed, time-resolved photoacoustic calorimetry , 1985 .

[15]  L. Libertini,et al.  Analysis of photoacoustic waveforms using the nonlinear least squares method. , 1992, Biophysical chemistry.

[16]  E. Henry,et al.  Geminate recombination of carbon monoxide to myoglobin. , 1983, Journal of molecular biology.

[17]  S. Sligar,et al.  Nucleotide sequence of the Pseudomonas putida cytochrome P-450cam gene and its expression in Escherichia coli. , 1986, The Journal of biological chemistry.

[18]  S. Braslavsky,et al.  Triplet lifetime determination by laser-induced optoacoustic spectroscopy. benzophenone/iodide revisited , 1986 .

[19]  H Frauenfelder,et al.  Dynamics of ligand binding to myoglobin. , 1975, Biochemistry.

[20]  S. Sligar,et al.  Role of the arginine-45 salt bridge in ligand dissociation from sperm whale carboxymyoglobin as probed by photoacoustic calorimetry. , 1990, Biochemistry.

[21]  C. Patel,et al.  Pulsed optoacoustic spectroscopy of condensed matter , 1981 .

[22]  S. Stanley,et al.  The Serine-rich Entamoeba histolytica Protein Is a Phosphorylated Membrane Protein Containing O-Linked Terminal N-Acetylglucosamine Residues (*) , 1995, The Journal of Biological Chemistry.

[23]  S. Sligar,et al.  Electrostatic control of the substrate access channel in cytochrome P-450cam. , 1994, Biochemistry.

[24]  Edward M. Eyring,et al.  Pulse shapes of nanosecond photoacoustic signals in liquids detected by piezoelectric foil , 1987 .

[25]  S. Boxer,et al.  Ligand and proton exchange dynamics in recombinant human myoglobin mutants. , 1989, Journal of molecular biology.

[26]  K. Peters,et al.  Photoacoustic calorimetric study of the conversion of rhodopsin and isorhodopsin to lumirhodopsin. , 1991, Biochemistry.

[27]  J B Callis,et al.  Fast changes of enthalpy and volume on flash excitation of Chromatium chromatophores. , 1972, Biochimica et biophysica acta.

[28]  A. Tam.  Applications of photoacoustic sensing techniques , 1986 .

[29]  S. Sligar,et al.  Catalytic mechanism of cytochrome P-450: evidence for a distal charge relay , 1992 .

[30]  K. Peters,et al.  Time-resolved photoacoustic calorimetry: a study of myoglobin and rhodopsin. , 1991, Annual review of biophysics and biophysical chemistry.