Ionic and osmotic influence on prostaglandin release from the gill tissue of a marine bivalve, Modiolus demissus.

1. Prostaglandin E2 (PGE2) was identified in Modiolus demissus gill tissue on the basis of solvent extraction, thin layer and column chromatography, bioassay, and radioimmunoassay. The presence of PGE2 was detected in both tissue and sea water incubate surrounding the tissue. 2. Both hyposmotic stress and magnesium-free sea water significantly increased release of prostaglandins into sea water. Hyposmotic stress also significantly increased prostaglandin synthesis. 3. Examination of tissues revealed that homogenates of the mantle and lower visceral mass contained significantly fewer nanograms immunoreactive prostaglandins per gram wet weight than homogenates of the gill, posterior adductor muscle, upper visceral mass, or siphon tissue. 4. Prostaglandin release could be increased by addition of arachidonic acid, and inhibited by addition of acetylsalicylic acid or indomethacin.

[1]  D. Morse,et al.  Capacity for biosynthesis of prostaglandin-related compounds: distribution and properties of the rate-limiting enzyme in hydrocorals, gorgoniants, and other coelenterates of the Caribbean and Pacific. , 1978, The Biological bulletin.

[2]  W. J. Hall,et al.  A role for endogenous prostaglandins in the short‐circuit current responses to osmolal changes in isolated frog skin , 1977 .

[3]  D. Morse,et al.  Hydrogen peroxide induces spawning in mollusks, with activation of prostaglandin endoperoxide synthetase. , 1977, Science.

[4]  U. E. Brady,et al.  Partial characterization of prostaglandin synthetase in the reproductive tract of the male house cricket, Acheta domesticus. , 1976, Prostaglandins.

[5]  H. Ogata,et al.  Distribution of prostaglandins in the animal kingdom. , 1976, Biochimica et biophysica acta.

[6]  R. Flower.  Drugs which inhibit prostaglandin biosynthesis. , 1974, Pharmacological reviews.

[7]  M. Greenberg,et al.  The Initiation and Control of Free Amino Acid Regulation of Cell Volume in Salinity-Stressed Marine Bivalves , 1973 .

[8]  E. Corey,et al.  Studies on the prostaglandin A 2 synthetase complex from Plexaura homomalla. , 1973, Journal of the American Chemical Society.

[9]  D. V. van Dorp,et al.  Comparative aspects of prostaglandin biosynthesis in animal tissues. , 1972, Biochimica et biophysica acta.

[10]  L. Wilkens.  Electrophysiological studies on the heart of the bivalve mollusc, Modiolus demissus. II. Ionic basis of the action potential. , 1972, The Journal of experimental biology.

[11]  L. Wilkens.  Electrophysiological studies on the heart of the bivalve mollusc, Modiolus demissus. I. Ionic basis of the membrane potential. , 1972, The Journal of experimental biology.

[12]  J. Vane,et al.  THE RELEASE OF PROSTAGLANDINS FROM LUNG AND OTHER TISSUES , 1971, Annals of the New York Academy of Sciences.

[13]  W. Lands,et al.  FACTORS REGULATING THE BIOSYNTHESIS OF VARIOUS PROSTAGLANDINS , 1971, Annals of the New York Academy of Sciences.

[14]  C. Sih,et al.  Mechanism of prostaglandin biosynthesis. 3. Catecholamines and serotonin as coenzymes. , 1970, Journal of the American Chemical Society.

[15]  S. Pierce.  The water balance of Modiolus (Mollusca: Bivalvia: Mytilidae): Osmotic concentrations in changing salinities , 1970 .

[16]  A. L. Willis.  Simplified thin-layer chromatography of prostaglanding in biological extracts. , 1970, British journal of pharmacology.

[17]  D Rodbard,et al.  Statistical quality control of radioimmunoassays. , 1968, The Journal of clinical endocrinology and metabolism.

[18]  J. Gougoutas,et al.  The identification of prostaglandins E2, F2α and A2 from rabbit kidney medulla , 1967 .

[19]  F. Coceani,et al.  Effect of nerve stimulation on prostaglandin formation and release from the rat stomach. , 1967, The American journal of physiology.

[20]  F. Coceani,et al.  On the action of prostaglandin E1 and prostaglandins from brain on the isolated rat stomach. , 1966, Canadian journal of physiology and pharmacology.

[21]  W. Vogt.  Pharamacologically active acidic phospholipids and glycolipids. , 1963, Biochemical pharmacology.

[22]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.