论文信息 - Rapid Immunostain for Demonstrating Estrogen Receptors on Paraffin Sections for Semiquantitation by Image Analysis

Rapid Immunostain for Demonstrating Estrogen Receptors on Paraffin Sections for Semiquantitation by Image Analysis

AbstractA rapid (2.25 hr) immunostain for estrogen receptor semiquantitation by image analysis is described. The immunostain utilized Abbott's ER-ICA Primary Antibody and the Code-On robotic irnrnunostainer. The image analysis was done with the Batchelor System Percent Activity Analysis software package utilizing the Optimas image analysis engine and Meyer Instruments equipment.The robotic immunostainer allowed precise control of time and temperature, facilitating reproducibility of the immunostain and semiquantitative measurement of estrogen receptor. The immunostain is an alkaline phosphatase method that is suitable for all specimens, notjust those requiring image analysis semiquantitation. (The J Histotechnol 17:47, 1994)

J. Lynn | B. Whitaker

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