Enhancing traveling-wave dielectrophoresis with signal superposition.

In this article, a new method for cell separation and characterization and for monitoring cell physiological changes was described. In this approach termed superposition-TWD (travelling-wave dielectrophoresis), one or more DEP and TWD signals are applied together. The effect of such superposition of signals is to change the levitation height of the particles above the electrode plane, and in so doing to alter the range of frequencies over which TWD occurs. Appropriate choices of TWD signal strengths and frequencies, as well as the senses of the applied quadrature phase sequences, can result in cells of different type or physiological state traveling in opposite directions. This provides significant advantages over previously described TWD methods and can result in improved levels of attainable sensitivity and purity of cell separations on shorter electrode tracks.

[1]  Ronald Pethig,et al.  Dielectrophoretic forces on particles in travelling electric fields , 1996 .

[2]  Ronald Pethig,et al.  Dielectrophoretic studies of the activation of human T lymphocytes using a newly developed cell profiling system , 2002, Electrophoresis.

[3]  G. Fuhr,et al.  Traveling‐wave dielectrophoresis of microparticles , 1992, Electrophoresis.

[4]  Julian P.H. Burt,et al.  A combined travelling wave dielectrophoresis and electrorotation device: applied to the concentration and viability determination of Cryptosporidium , 1997 .

[5]  F. Becker,et al.  A unified theory of dielectrophoresis and travelling wave dielectrophoresis , 1994 .

[6]  R. Pethig,et al.  Electromanipulation and separation of cells using travelling electric fields , 1996 .

[7]  F F Becker,et al.  Dielectrophoretic manipulation of cells with spiral electrodes. , 1997, Biophysical journal.

[8]  Ronald Pethig,et al.  Development of biofactory-on-a-chip technology using excimer laser micromachining , 1998 .

[9]  F F Becker,et al.  Cell separation on microfabricated electrodes using dielectrophoretic/gravitational field-flow fractionation. , 1999, Analytical chemistry.

[10]  R. Pethig,et al.  Microelectrode devices for manipulating and analysing bioparticles , 1998 .

[11]  M. Washizu,et al.  Movement of Blood Cells in Liquid by Non-Uniform Travelling Field , 1986, 1986 Annual Meeting Industry Applications Society.

[12]  Y. Huang,et al.  Electrokinetic behaviour of colloidal particles in travelling electric fields: studies using yeast cells , 1993 .

[13]  Ronald Pethig,et al.  The dielectrophoretic levitation of latex beads, with reference to field-flow fractionation , 1997 .

[14]  Ulrich Zimmermann,et al.  Electro-rotation: development of a technique for dielectric measurements on individual cells and particles , 1988 .

[15]  Hywel Morgan,et al.  Large-area travelling-wave dielectrophoresis particle separator , 1997 .

[16]  H. Morgan,et al.  The dielectrophoretic and travelling wave forces generated by interdigitated electrode arrays: analytical solution using Fourier series , 2001 .