MoonProt 2.0: an expansion and update of the moonlighting proteins database

Abstract MoonProt 2.0 (http://moonlightingproteins.org) is an updated, comprehensive and open-access database storing expert-curated annotations for moonlighting proteins. Moonlighting proteins contain two or more physiologically relevant distinct functions performed by a single polypeptide chain. Here, we describe developments in the MoonProt website and database since our previous report in the Database Issue of Nucleic Acids Research. For this V 2.0 release, we expanded the number of proteins annotated to 370 and modified several dozen protein annotations with additional or updated information, including more links to protein structures in the Protein Data Bank, compared with the previous release. The new entries include more examples from humans and several model organisms, more proteins involved in disease, and proteins with different combinations of functions. The updated web interface includes a search function using BLAST to enable users to search the database for proteins that share amino acid sequence similarity with a protein of interest. The updated website also includes additional background information about moonlighting proteins and an expanded list of links to published articles about moonlighting proteins.

[1]  Deyou Zheng,et al.  Gene sharing and evolution , 2009, Human Genomics.

[2]  Sheng Wang,et al.  Proteomics: a powerful tool to study plant responses to biotic stress , 2019, Plant Methods.

[3]  I. Wool.  Extraribosomal functions of ribosomal proteins. , 1996, Trends in biochemical sciences.

[4]  Thomas L. Madden,et al.  BLAST: at the core of a powerful and diverse set of sequence analysis tools , 2004, Nucleic Acids Res..

[5]  The Gene Ontology Consortium,et al.  Expansion of the Gene Ontology knowledgebase and resources , 2016, Nucleic Acids Res..

[6]  Carlos Gancedo,et al.  Moonlighting Proteins in Yeasts , 2008, Microbiology and Molecular Biology Reviews.

[7]  Constance J Jeffery,et al.  Moonlighting proteins: old proteins learning new tricks. , 2003, Trends in genetics : TIG.

[8]  Enrique Querol,et al.  MultitaskProtDB: a database of multitasking proteins , 2013, Nucleic Acids Res..

[9]  The Gene Ontology Consortium.  Expansion of the Gene Ontology knowledgebase and resources , 2016, Nucleic Acids Res..

[10]  J. Stülke,et al.  Trigger enzymes: bifunctional proteins active in metabolism and in controlling gene expression , 2007, Molecular microbiology.

[11]  A. Graham Pockley,et al.  Molecular chaperones and protein‐folding catalysts as intercellular signaling regulators in immunity and inflammation , 2010, Journal of leukocyte biology.

[12]  Constance J Jeffery,et al.  Moonlighting proteins--an update. , 2009, Molecular bioSystems.

[13]  Paul Schimmel,et al.  Essential nontranslational functions of tRNA synthetases. , 2013, Nature chemical biology.

[14]  Petri Ihalainen,et al.  Structural and Functional Dynamics of Staphylococcus aureus Biofilms and Biofilm Matrix Proteins on Different Clinical Materials , 2019, Microorganisms.

[15]  Brian Henderson,et al.  Bacterial Virulence in the Moonlight: Multitasking Bacterial Moonlighting Proteins Are Virulence Determinants in Infectious Disease , 2011, Infection and Immunity.

[16]  Cathy H. Wu,et al.  UniProt: the Universal Protein knowledgebase , 2004, Nucleic Acids Res..

[17]  J. Piatigorsky,et al.  Recruitment of enzymes as lens structural proteins. , 1987, Science.

[18]  Constance Jeffery,et al.  Moonlighting proteins , 2010, Genome Biology.

[19]  Haipeng Liu,et al.  MoonProt: a database for proteins that are known to moonlight , 2013, Nucleic Acids Res..

[20]  David S. Goodsell,et al.  The RCSB protein data bank: integrative view of protein, gene and 3D structural information , 2016, Nucleic Acids Res..