Nanoshells as contrast agents for scatter-based optical imaging

Optical properties of gold nanoshell-based contrast agents for optical coherence tomography (OCT) were determined using calculations based on concentric sphere Mie scattering theory. Nanoshells capable of optimal optical scattering in the near infrared (NIR) were subsequently fabricated. We show that scattering nanoshell possess higher backscattering efficiencies at longer wavelengths. Results show enhanced OCT signal intensities after addition of gold nanoshells to scattering tissue phantoms. In addition, in vivo mouse tumor model studies show clear evidence of enhancement of tumor scatter after nanoshell injection as compared to control (phosphate buffered saline, PBS) injections, further demonstrating the potential of gold nanoshells as NIR contrast agents to improve scattering-based optical imaging technologies. In addition to the data shown in this preliminary paper, the talk will present more recent data using immunotargeted nanoshells for molecular contrast OCT

[1]  Z. Kam,et al.  Absorption and Scattering of Light by Small Particles , 1998 .

[2]  K. Suslick,et al.  Engineered microsphere contrast agents for optical coherence tomography. , 2003, Optics letters.

[3]  F. Capasso,et al.  Differential near-field scanning optical microscopy , 2007, Quantum Electronics and Laser Science Conference.

[4]  J. West,et al.  Immunotargeted nanoshells for integrated cancer imaging and therapy. , 2005, Nano letters.

[5]  Qianfan Xu,et al.  Experimental demonstration of guiding and confining light in nanometer-size low-refractive-index material. , 2004, Optics letters.

[6]  Andreas Tycho,et al.  Extraction of optical scattering parameters and attenuation compensation in optical coherence tomography images of multilayered tissue structures. , 2004, Optics letters.

[7]  Peter P. Edwards,et al.  A new hydrosol of gold clusters. 1. Formation and particle size variation , 1993 .

[8]  Leon Hirsch,et al.  Nanoshell-Enabled Photonics-Based Imaging and Therapy of Cancer , 2004, Technology in cancer research & treatment.

[9]  Leon Hirsch,et al.  Gold nanoshell bioconjugates for molecular imaging in living cells. , 2005, Optics letters.

[10]  Naomi J. Halas,et al.  Nanoengineering of optical resonances , 1998 .

[11]  G. Gelikonov,et al.  In vivo endoscopic OCT imaging of precancer and cancer states of human mucosa. , 1997, Optics express.

[12]  Jennifer Kehlet Barton,et al.  Use of microbubbles as an optical coherence tomography contrast agent. , 2002, Academic radiology.

[13]  R. Stafford,et al.  Nanoshell-mediated near-infrared thermal therapy of tumors under magnetic resonance guidance , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[14]  J. Fujimoto,et al.  Optical coherence tomography for optical biopsy. Properties and demonstration of vascular pathology. , 1996, Circulation.

[15]  J. Fujimoto,et al.  Optical Coherence Tomography , 1991 .

[16]  J. Izatt,et al.  In vivo bidirectional color Doppler flow imaging of picoliter blood volumes using optical coherence tomography. , 1997, Optics letters.

[17]  Naomi J. Halas,et al.  Relative contributions to the plasmon line shape of metal nanoshells , 2002 .