Treatment of Ballast Water; How to Test a System with a Modular Concept?

A variety of methods were successfully applied to examine the efficacy of a modular ballast water system according to the standards as adopted by the International Maritime Organization. The ballast water treatment system had a capacity of 530 m3 h−1 consisted of a pump system, a hydrocyclone, a 50 μm mesh-size self-cleaning filter and an installation for the addition of a chemical disinfectant (PERACLEAN® Ocean). The land-based testing facility used natural sea water of high turbidity during the spring phytoplankton bloom. The mesozooplankton fraction was inspected with a standard binocular. Larger zooplankton were effectively removed with the filter; the smaller sized fraction containing larvae and nauplia were killed after chemical treatment. The phytoplankton component was monitored using flow cytometry. The huge colonies of the phytoplankton Phaeocystis globosa were disrupted in the hydrocyclone liberating the colony cells which passed as single cells through the filter. These cells remained viable but were finally killed in the secondary (chemical) step. Bacteria also passed all mechanical treatment steps unharmed but were killed in the final step. Viability tests with SYTOX Green, which were specifically designed for phytoplankton, showed that mechanical treatment did not affect the percentage of viable cells a short-term, but after several hours the viable cell counts dropped down to 70%. Phytoplankton cells recovered within a single day and formed a new dense bloom rapidly. The bacteriostatic component of the chemical disinfectant (H2O2) remained present for several days preventing regrowth of bacteria for up to 15 days after addition. In conclusion, the IMO standards were met using the modular ballast water treatment unit and the applied instruments and assays were effective and rapid tools to qualify and quantify the organisms present as well as their viability.

[1]  M. Gauns,et al.  A first report on a bloom of the marine prymnesiophycean, Phaeocystis globosa from the Arabian Sea , 2000 .

[2]  J. Geller,et al.  Ecological Roulette: The Global Transport of Nonindigenous Marine Organisms , 1993, Science.

[3]  P. Burkill,et al.  The rapid analysis of single marine cells by flow cytometry , 1990, Philosophical Transactions of the Royal Society of London. Series A: Physical and Engineering Sciences.

[4]  M. Veldhuis,et al.  Cell death in phytoplankton: correlation between changes in membrane permeability, photosynthetic activity, pigmentation and growth , 2001 .

[5]  William K. W. Li,et al.  DNA distributions in planktonic bacteria stained with TOTO or TO‐PRO , 1995 .

[6]  Huang Chang-jian.  TAXONOMIC AND ECOLOGICAL STUDIES ON A LARGE SCALE PHAEOCYSTIS POUCHETII BLOOM IN THE SOUTHEAST COAST OF CHINA DURING LATE 1997 , 1999 .

[7]  E. V. Wal,et al.  Cargo vessel ballast water as a vector for the transport of non-indigenous marine species , 1988 .

[8]  M. Veldhuis,et al.  Application of flow cytometry in marine phytoplankton research: current applications and future perspectives , 2000 .

[9]  F. Dobbs,et al.  Microbial ecology of ballast water during a transoceanic voyage and the effects of open-ocean exchange , 2002 .

[10]  Marcel J. W. Veldhuis,et al.  Phaeocystis blooms and nutrient enrichment in the continental coastal zones of the North sea , 1987 .

[11]  U. Karst,et al.  Selective photometric determination of peroxycarboxylic acids in the presence of hydrogen peroxide , 1997 .

[12]  G. Cadée.  Tidal and seasonal variation in particulate and dissolved organic carbon in the western dutch Wadden Sea and Marsdiep tidal inlet , 1982 .

[13]  G. Bratbak,et al.  Flow cytometric detection of viruses. , 2000, Journal of virological methods.

[14]  Christiane Lancelot,et al.  Phaeocystis blooms in the global ocean and their controlling mechanisms: a review , 2005 .

[15]  R. Jonker,et al.  Flow cytometry as a tool for the study of phytoplankton , 2000 .

[16]  P. Hoagland,et al.  The economic effects of harmful algal blooms in the United States: Estimates, assessment issues, and information needs , 2002 .

[17]  Robert C. Wolpert,et al.  A Review of the , 1985 .

[18]  B. Dale,et al.  Harmful algal blooms in European marine and brackish waters , 1999 .

[19]  M. Veldhuis,et al.  Phytoplankton in the subtropical Atlantic Ocean: towards a better assessment of biomass and composition , 2004 .

[20]  B. Mitchell,et al.  Mass sedimentation of Phaeocystis pouchetii in the Barents Sea , 1990 .

[21]  Rolf Skjong,et al.  Challenges in global ballast water management. , 2004, Marine pollution bulletin.

[22]  Rita R. Colwell.  Global Climate and Infectious Disease: The Cholera Paradigm* , 1996, Science.

[23]  M. C. Villac,et al.  Species Richness and Invasion Vectors: Sampling Techniques and Biases , 2004, Biological Invasions.

[24]  Rita R. Colwell,et al.  Global spread of microorganisms by ships , 2000, Nature.

[25]  Gustaaf M. Hallegraeff,et al.  Temperature tolerances of toxic dinoflagellate cysts: application to the treatment of ships' ballast water , 1997, Aquatic Ecology.

[26]  F. Dobbs,et al.  Global Redistribution of Bacterioplankton and Virioplankton Communities , 2001, Biological Invasions.

[27]  J. Hamer,et al.  Dinoflagellate cysts in ballast tank sediments : Between tank variability , 2000 .

[28]  James T. Carlton,et al.  Remarkable invasion of San Francisco Bay (California, USA), by the Asian clam Potamocorbula amurensis. I. Introduction and dispersal , 1990 .

[29]  M. Veldhuis,et al.  Bloom dynamics and biological control of a high biomass HAB species in European coastal waters: A Phaeocystis case study , 2005 .

[30]  Josep M. Gasol,et al.  Using flow cytometry for counting natural planktonic bacteria and understanding the structure of planktonic bacterial communities , 2000 .

[31]  M. Poot,et al.  Bacterial viability and antibiotic susceptibility testing with SYTOX green nucleic acid stain , 1997, Applied and environmental microbiology.