Alteration in carbon partitioning induced by the movement protein of tobacco mosaic virus originates in the mesophyll and is independent of change in the plasmodesmal size exclusion limit

The influence of the 30 kDa movement protein of tobacco mosaic virus (TMV-MP) on carbon partitioning in trans-genie tobacco plants (Nicotiana tabacum cv. Xanthi) expressing the TMV-MP was investigated. Using reciprocal grafting of transgenic tobacco plants expressing this movement protein and vector control plants, as well as transgenic tobacco plants expressing the TMV-MP in phloem cells only, we showed that the interactive site involved in carbon allocation to roots is localized to the mesophyll tissue. Biomass partitioning experiments conducted on transgenic plants, in which various deletion mutant forms of the TMV-MP (two of which included deletions in the domain responsible for increasing the size exclusion limit) were expressed, revealed that the TMV-MP exerts its influence on carbon allocation via a mechanism that is completely independent of the TMV-MP-induced increase in the plasmodesmal size exclusion limit. Furthermore, small N- and C-terminal deletions in the MP revealed the complexity of the interactions likely to be involved between the MP and an endogenous regulatory mechanism. We propose that the TMV-MP interferes with an endogenous signal transduction pathway that involves macromolecular trafficking through plasmodesmata to regulate biomass partitioning between the source and various sink tissues.

[1]  W. J. Lucas,et al.  Two proteins of a plant DNA virus coordinate nuclear and plasmodesmal transport , 1994, Cell.

[2]  W. J. Lucas,et al.  Direct functional assay for tobacco mosaic virus cell-to-cell movement protein and identification of a domain involved in increasing plasmodesmal permeability. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[3]  W. J. Lucas,et al.  Cell-to-Cell Trafficking of Macromolecules through Plasmodesmata Potentiated by the Red Clover Necrotic Mosaic Virus Movement Protein. , 1993, The Plant cell.

[4]  W. J. Lucas,et al.  A dysfunctional movement protein of tobacco mosaic virus that partially modifies the plasmodesmata and limits virus spread in transgenic plants , 1993 .

[5]  W. J. Lucas,et al.  Plasmodesmata: the intercellular organelles of green plants. , 1993, Trends in cell biology.

[6]  R. Beachy,et al.  Coat protein-mediated resistance in transgenic tobacco expressing the tobacco mosaic virus coat protein from tissue-specific promoters. , 1993, Molecular plant-microbe interactions : MPMI.

[7]  D. B. Fisher,et al.  Turnover of soluble proteins in the wheat sieve tube. , 1992, Plant physiology.

[8]  W. J. Lucas,et al.  Secondary plasmodesmata are specific sites of localization of the tobacco mosaic virus movement protein in transgenic tobacco plants. , 1992, The Plant cell.

[9]  R. Beachy,et al.  Effects of terminal deletion mutations on function of the movement protein of tobacco mosaic virus. , 1992, Virology.

[10]  W. J. Lucas,et al.  The TMV movement protein: role of the C-terminal 73 amino acids in subcellular localization and function. , 1991, Virology.

[11]  W. J. Lucas,et al.  Plasmodesmatal function is probed using transgenic tobacco plants that express a virus movement protein. , 1991, The Plant cell.

[12]  C. Raper,et al.  Soluble Carbohydrate Allocation to Roots, Photosynthetic Rate of Leaves, and Nitrate Assimilation as Affected by Nitrogen Stress and Irradiance , 1991, Botanical Gazette.

[13]  I. F. Wardlaw,et al.  Tansley Review No. 27 The control of carbon partitioning in plants. , 1990, The New phytologist.

[14]  S. Britz.  PHOTOREGULATION OF ROOT: SHOOT RATIO IN SOYBEAN SEEDLINGS * , 1990 .

[15]  W. J. Lucas,et al.  Movement Protein of Tobacco Mosaic Virus Modifies Plasmodesmatal Size Exclusion Limit , 1989, Science.

[16]  C. M. Deom,et al.  The 30-Kilodalton Gene Product of Tobacco Mosaic Virus Potentiates Virus Movement , 1987, Science.

[17]  H. Towbin,et al.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[18]  W. J. Lucas,et al.  Plasmodesmata in Relation to Viral Movement within Leaf Tissues , 1994 .

[19]  W. J. Lucas,et al.  Altered function of the tobacco mosaic virus movement protein in a hypersensitive host. , 1991, Virology.

[20]  H. Mooney,et al.  6 – Partitioning Response of Plants to Stress , 1991 .

[21]  William J. Davies,et al.  Root Signals and the Regulation of Growth and Development of Plants in Drying Soil , 1991 .

[22]  D. Geiger,et al.  Carbon Allocation and Response to Stress , 1984 .