THE INFLUENCE OF MERCURY ON COMPUTER ANALYZED SPERM MOTILITY OF COMMON CARP, CYPRINUS CARPIO L., IN VITRO

The aim of this work was to evaluate the impact of different mercury concentrations (ranging from 0.05 to 1 ppm) on common carp sperm motility. Computer assisted sperm analysis (CASA) and microscopic observations of the time of sperm motility were applied in the experiment. According to analysis using the CASA method, it was found that all the tested concentrations of mercury decreased sperm motility, and that lethal effects were observed at concentrations of 0.5 and 1 ppm. On the other hand, microscopic observations showed that mercury decreased the time of sperm movement at doses as low as 0.2 ppm, and that lethal effects were evident at 1ppm. These results suggest the negative impact of mercury on sperm motility. The reasons for the differences in the results obtained using these two techniques are also discussed.

[1]  D. Kime,et al.  The influence of zinc on sperm motility of common carp - a computer assisted studies , 2000 .

[2]  D. Kime.  Endocrine Disruption in Fish , 1998, Springer US.

[3]  D. Kime,et al.  Use of computer assisted sperm analysis (CASA) for monitoring the effects of pollution on sperm quality of fish; Application to the effects of heavy metals , 1996 .

[4]  G. Centola.  Comparison of manual microscopic and computer-assisted methods for analysis of sperm count and motility. , 1996, Archives of andrology.

[5]  J. Torsella,et al.  Computer‐assisted motion analysis of sperm from the common carp , 1995 .

[6]  P. Allen.  Distribution of mercury in the soft tissues of the blue tilapia Oreochromis aureus (Steindachner) after acute exposure to mercury (II) chloride , 1994, Bulletin of environmental contamination and toxicology.

[7]  M. Fournier,et al.  In vitro mercury-related cytotoxicity and functional impairment of the immune cells of rainbow trout (Oncorhynchus mykiss) , 1994 .

[8]  K. Gwoździński.  Structural changes of proteins in fish red blood cells after copper and mercury treatment , 1992, Archives of environmental contamination and toxicology.

[9]  J. Nielsen.  Toxicokinetics of mercuric chloride and methylmercuric chloride in mice. , 1992, Journal of toxicology and environmental health.

[10]  R. Kirubagaran,et al.  Toxic effects of mercury on testicular activity in the freshwater teleost, Clarias batrachus (L.) , 1992 .

[11]  R. Billard,et al.  Some problems related to the assessment of sperm motility in freshwater fish , 1992 .

[12]  M. Protasowicki.  Long term studies on heavy metals in aquatic organisms from the River Odra mouth area , 1991 .

[13]  S. Dey,et al.  Ovarian damage to Channa punctatus after chronic exposure to low concentrations of Elsan, mercury, and ammonia. , 1989, Ecotoxicology and environmental safety.

[14]  R. Kirubagaran,et al.  An immunocytochemical study on the pituitary gonadotropic and thyrotropic cells in the catfish, Clarias batrachus after mercury treatment , 1989 .

[15]  R. Kirubagaran,et al.  Toxic effects of mercuric chloride, methylmercuric chloride, and emisan 6 (an organic mercurial fungicide) on ovarian recrudescence in the catfishClarias batrachus (L.) , 1988, Bulletin of environmental contamination and toxicology.

[16]  J. Weis,et al.  Effects of methylmercury on sperm and egg viability of two populations of killifish (fundulus heteroclitus) , 1987, Archives of environmental contamination and toxicology.

[17]  J. Weis,et al.  Toxic effects of mercuric chloride on sperm and egg viability of two populations of mummichog, Fundulus heteroclitus. , 1987, Environmental pollution.

[18]  T. Burbacher,et al.  Effects of methyl mercury on sperm oxygen consumption. , 2009, Acta pharmacologica et toxicologica.

[19]  L. Tollefson,et al.  Methylmercury in fish: a review of residue levels, fish consumption and regulatory action in the United States. , 1986, Environmental health perspectives.

[20]  P. Roubaud,et al.  The effect of metals and cyanide on fertilization in rainbow trout (Salmo gairdneri) , 1985 .

[21]  J. Heisinger,et al.  Mercuric chloride uptake by eggs of the ricefish and resulting teratogenic effects , 1975, Bulletin of environmental contamination and toxicology.

[22]  J. Mcintyre.  Toxicity of methyl mercury for steelhead trout sperm , 1973, Bulletin of environmental contamination and toxicology.