COMPARISON OF ANTIRADICAL ACTIVITY AND TOTAL PHENOLIC CONTENT IN SEEDS OF FIVE SOYBEAN CULTIVARS BY APPLYING DIFFERENT EXTRACTION SOLVENTS

Radical scavenging activity and total phenolic content in seeds of five Bulgarian soybean varieties were analyzed in relation to different types of the extraction solvent. The extraction solvents used were acetonitrile acidified with HCl and 80% methanol. The amount of phenolic compounds in the extracts was estimated by Folin–Ciocalteu reagent. 2,2-diphenyl1-picryl hydrazyl (DPPH) radicals were used for evaluation of antiradical activity. Statistically significant differences in respect to total phenolic content and antiradical activity were not established among the studied methanol and acetonitrile extracts of one and the same variety. Total phenolic content in soybean cultivars ranged from 7.52 to 12.87 mg GAE/g extract. The cultivars “Rosa”, “Richy” and “Daniela” showed higher levels of phenols. Antiradical activity expressed as the concentration of extracts needed for 50 % inhibition of radicals (IC50) ranged from 2.74 to 3.84 mg/mL. The results from the comparative analysis show that methanol and acetonitrile are interchangeable extraction solvents for determination of antiradical activity and total phenolic content in soybean samples.

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