Biological pretreatment of rice straw by fermenting with Dichomitus squalens.

Rice straw was fermented by a wood-rot fungus Dichomitus squalens as a biological pretreatment, to increase the enzymatic digestibility of lignocellulose and promote cellulose hydrolysis. Response surface methodology was employed to optimize the fermentation medium of D. squalens for achieving the maximum volumetric activity of manganese peroxidase. The fermentation of rice straw by D. squalens for 15 days resulted in the enzymatic digestibility of 58.1% of theoretical glucose yield for the remaining glucan. In addition, a significant reduction in the crystallinity index and microstructural changes in the fermented rice straw were revealed. When the fungal-fermented rice straw was used as a substrate for ethanol production in simultaneous saccharification and fermentation, the ethanol production yield and productivity were 54.2% of the theoretical maximum and 0.39 g/L/hour, respectively, after 24 hours.

[1]  Robinson,et al.  Fermentability of the hemicellulose-derived sugars from steam-exploded softwood (douglas fir) , 1999, Biotechnology and bioengineering.

[2]  M. Tien,et al.  Stimulation of Mn peroxidase activity: a possible role for oxalate in lignin biodegradation. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[3]  J. W. Koenigs.  Hydrogen Peroxide and Iron: A Proposed System for Decomposition of Wood by Brown-rot Basidiomycetes , 1974 .

[4]  V. Bisaria,et al.  Simultaneous bioconversion of cellulose and hemicellulose to ethanol. , 1998, Critical reviews in biotechnology.

[5]  Breen,et al.  Fungi in lignocellulose breakdown and biopulping , 1999, Current opinion in biotechnology.

[6]  G. L. Miller.  Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar , 1959 .

[7]  T. K. Ghose.  Measurement of cellulase activities , 1987 .

[8]  T. Lundell,et al.  Production of organic acids and oxalate decarboxylase in lignin-degrading white rot fungi , 2002 .

[9]  E. Lohmeier-Vogel,et al.  Intracellular acidification as a mechanism for the inhibition by acid hydrolysis-derived inhibitors of xylose fermentation by yeasts , 1998, Journal of Industrial Microbiology and Biotechnology.

[10]  T. Jeffries.  Biodegradation of lignin-carbohydrate complexes , 1990, Biodegradation.

[11]  H. Taguchi,et al.  Structural properties of cellulose and cellulase reaction mechanism , 1983, Biotechnology and bioengineering.

[12]  Norman R. Draper,et al.  "RIDGE ANALYSIS" OF RESPONSE SURFACES , 1963 .

[13]  S. Khanal,et al.  Solid-substrate fermentation of corn fiber by Phanerochaete chrysosporium and subsequent fermentation of hydrolysate into ethanol. , 2008, Journal of agricultural and food chemistry.

[14]  Ye Sun,et al.  Hydrolysis of lignocellulosic materials for ethanol production: a review. , 2002, Bioresource technology.

[15]  F. Guillén,et al.  Substrate specificity and properties of the aryl-alcohol oxidase from the ligninolytic fungus Pleurotus eryngii. , 1992, European journal of biochemistry.

[16]  U. Urzúa,et al.  Manganese Peroxidase-Dependent Oxidation of Glyoxylic and Oxalic Acids Synthesized by Ceriporiopsis subvermispora Produces Extracellular Hydrogen Peroxide , 1998, Applied and Environmental Microbiology.

[17]  M. M. Gharpuray,et al.  Structural modification of lignocellulosics by pretreatments to enhance enzymatic hydrolysis , 1983, Biotechnology and bioengineering.

[18]  R. Plackett,et al.  THE DESIGN OF OPTIMUM MULTIFACTORIAL EXPERIMENTS , 1946 .

[19]  M. Tien,et al.  Lignin-Degrading Enzyme from the Hymenomycete Phanerochaete chrysosporium Burds , 1983, Science.

[20]  Y. Y. Lee,et al.  Pretreatment of corn stover by soaking in aqueous ammonia , 2005, Applied biochemistry and biotechnology.

[21]  M. Leisola,et al.  An extracellular aryl-alcohol oxidase from the white-rot fungus Bjerkandera adusta , 1990 .

[22]  R. Tengerdy,et al.  Bioconversion of lignocellulose in solid substrate fermentation , 2003 .

[23]  P. Lebaron,et al.  Comparison of Blue Nucleic Acid Dyes for Flow Cytometric Enumeration of Bacteria in Aquatic Systems , 1998, Applied and Environmental Microbiology.

[24]  R. Bourbonnais,et al.  Veratryl alcohol oxidases from the lignin-degrading basidiomycete Pleurotus sajor-caju. , 1988, The Biochemical journal.

[25]  Cady R. Engler,et al.  Effects of cell-wall acetate, xylan backbone, and lignin on enzymatic hydrolysis of aspen wood , 1992 .

[26]  Takashi Sasaki,et al.  Correlation between X‐ray diffraction measurements of cellulose crystalline structure and the susceptibility to microbial cellulase , 1979 .

[27]  Sandy Merino,et al.  Progress and challenges in enzyme development for biomass utilization. , 2007, Advances in biochemical engineering/biotechnology.

[28]  Jian Shi,et al.  EFFECT OF MICROBIAL PRETREATMENT ON ENZYMATIC HYDROLYSIS AND FERMENTATION OF COTTON STALKS FOR ETHANOL PRODUCTION , 2009 .

[29]  In-Geol Choi,et al.  Improved enzymatic hydrolysis yield of rice straw using electron beam irradiation pretreatment. , 2009, Bioresource technology.

[30]  W. G. Glasser,et al.  Lignin Impact on Fiber Degradation. 3. Reversal of Inhibition of Enzymatic Hydrolysis by Chemical Modification of Lignin and by Additives , 1997 .

[31]  Q. Nguyen,et al.  Microbial pretreatment of biomass , 2003, Applied biochemistry and biotechnology.

[32]  Douglas C. Montgomery,et al.  Response Surface Methodology: Process and Product Optimization Using Designed Experiments , 1995 .

[33]  D. S. Arora,et al.  Laccase production by some white rot fungi under different nutritional conditions , 2000 .

[34]  Armin Fiechter,et al.  Enzymes of white-rot fungi involved in lignin degradation and ecological determinants for wood decay , 1995 .

[35]  P. Kersten,et al.  Involvement of a new enzyme, glyoxal oxidase, in extracellular H2O2 production by Phanerochaete chrysosporium , 1987, Journal of bacteriology.

[36]  J. Field,et al.  2-Chloro-1,4-Dimethoxybenzene as a Novel Catalytic Cofactor for Oxidation of Anisyl Alcohol by Lignin Peroxidase , 1998, Applied and Environmental Microbiology.

[37]  F. Périé,et al.  Manganese regulation of manganese peroxidase expression and lignin degradation by the white rot fungus Dichomitus squalens , 1991, Applied and environmental microbiology.

[38]  E G Koukios,et al.  Correlating the effect of pretreatment on the enzymatic hydrolysis of straw , 1992, Biotechnology and bioengineering.

[39]  B. Dale,et al.  Enzymatic hydrolysis and recrystallization behavior of initially amorphous cellulose , 1985, Biotechnology and bioengineering.

[40]  H. Wariishi,et al.  Thiol-mediated oxidation of nonphenolic lignin model compounds by manganese peroxidase of Phanerochaete chrysosporium. , 1989, The Journal of biological chemistry.

[41]  Richard A Dixon,et al.  Lignin modification improves fermentable sugar yields for biofuel production , 2007, Nature Biotechnology.

[42]  R. Bourbonnais,et al.  Reactivities of various mediators and laccases with kraft pulp and lignin model compounds , 1997, Applied and environmental microbiology.

[43]  R. Farrell,et al.  Enzymatic "combustion": the microbial degradation of lignin. , 1987, Annual review of microbiology.

[44]  G. Daniel,et al.  Low molecular weight chelators and phenolic compounds isolated from wood decay fungi and their role in the fungal biodegradation of wood , 1997 .

[45]  M. Tien,et al.  Overproduction of lignin-degrading enzymes by an isolate of Phanerochaete chrysosporium , 1991, Applied and environmental microbiology.

[46]  A. Ragauskas,et al.  Enhanced enzymatic hydrolysis of spruce by alkaline pretreatment at low temperature , 2008, Biotechnology and bioengineering.

[47]  C. Wyman,et al.  Effect of xylan and lignin removal by batch and flowthrough pretreatment on the enzymatic digestibility of corn stover cellulose , 2004, Biotechnology and bioengineering.

[48]  Bryan Bals,et al.  Optimization of Ammonia Fiber Expansion (AFEX) Pretreatment and Enzymatic Hydrolysis of Miscanthus x giganteus to Fermentable Sugars , 2007, Biotechnology progress.