In-Depth High-Throughput Screening of Protein Engineering Libraries by Split-GFP Direct Crude Cell Extract Data Normalization.

[1]  B. Moss,et al.  Topology of Endoplasmic Reticulum‐Associated Cellular and Viral Proteins Determined with Split‐GFP , 2015, Traffic.

[2]  U. Bornscheuer,et al.  Engineering the Active Site of the Amine Transaminase from Vibrio fluvialis for the Asymmetric Synthesis of Aryl–Alkyl Amines and Amino Alcohols , 2015 .

[3]  U. Bornscheuer,et al.  An enzyme cascade synthesis of ε-caprolactone and its oligomers. , 2015, Angewandte Chemie.

[4]  M. Rose,et al.  Kar5p Is Required for Multiple Functions in Both Inner and Outer Nuclear Envelope Fusion in Saccharomyces cerevisiae , 2014, G3: Genes, Genomes, Genetics.

[5]  U. Bornscheuer,et al.  Glycine oxidase based high-throughput solid-phase assay for substrate profiling and directed evolution of (R)- and (S)-selective amine transaminases. , 2014, Analytical chemistry.

[6]  Ulrich Schwaneberg,et al.  To get what we aim for – progress in diversity generation methods , 2013, The FEBS journal.

[7]  G. Huisman,et al.  Engineering the third wave of biocatalysis , 2012, Nature.

[8]  Hau B. Nguyen,et al.  Experimental mapping of soluble protein domains using a hierarchical approach , 2011, Nucleic acids research.

[9]  A. Theberge,et al.  Microdroplets in microfluidics: an evolving platform for discoveries in chemistry and biology. , 2010, Angewandte Chemie.

[10]  Philip A. Romero,et al.  Exploring protein fitness landscapes by directed evolution , 2009, Nature Reviews Molecular Cell Biology.

[11]  S. Withers,et al.  Ultrahigh‐Throughput FACS‐Based Screening for Directed Enzyme Evolution , 2009, Chembiochem : a European journal of chemical biology.

[12]  Karen Robins,et al.  Rapid and sensitive kinetic assay for characterization of omega-transaminases. , 2009, Analytical chemistry.

[13]  E. Barnard,et al.  Detection and localisation of protein-protein interactions in Saccharomyces cerevisiae using a split-GFP method. , 2008, Fungal genetics and biology : FG & B.

[14]  Uwe T Bornscheuer,et al.  Isoenzymes of pig-liver esterase reveal striking differences in enantioselectivities. , 2007, Angewandte Chemie.

[15]  G. Waldo,et al.  In vivo and in vitro protein solubility assays using split GFP , 2006, Nature Methods.

[16]  Helena Berglund,et al.  Improved solubility of TEV protease by directed evolution. , 2006, Journal of biotechnology.

[17]  T. Terwilliger,et al.  Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein , 2005, Nature Biotechnology.

[18]  Thomas D. Y. Chung,et al.  A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays , 1999, Journal of biomolecular screening.

[19]  U. Bornscheuer,et al.  Characterization and enantioselectivity of a recombinant esterase from Pseudomonas fluorescens , 1998 .

[20]  P. Trudgill,et al.  The purification and properties of cyclohexanone oxygenase from Nocardia globerula CL1 and Acinetobacter NCIB 9871. , 1976, European journal of biochemistry.

[21]  K. Miyazaki MEGAWHOP cloning: a method of creating random mutagenesis libraries via megaprimer PCR of whole plasmids. , 2011, Methods in enzymology.

[22]  D. M. Taverna,et al.  Why are proteins marginally stable? , 2002, Proteins.