Antimicrobial Activity of Ethnobotanical Plant Psidium Guajava

The present work demonstrates the antimicrobial potential of Psidium guajava leaves extract by using various solvents. The results indicate that water and acetone are better than methanol and ether for the extraction of the antibacterial properties of guava. The results also indicate that the plant extracts have antibacterial effect on both Gram positive and Gram-negative bacteria, that is, can be used as broad spectrum antibacterial compounds. The observed inhibition of Gram-positive bacteria Staphylococcus aureus and Gram negative bacteria E.coli suggests that guava possesses compounds containing antibacterial properties that can effectively suppress the growth when extracted using acetone and water as solvent. Comparisons with related data from the literature indicate that according to the different methodologies of studies on antibacterial activity, the most diverse outcomes can be obtained. This study provides scientific insight to further determine the antimicrobial principles and investigate other pharmacological properties of guava. On the basis of the present finding, P. guajava leaves possess capabilities of being a good candidate in the search for a natural antimicrobial agent against infections and/or diseases caused by S. aureus and E.coli.

[1]  F. Jabin,et al.  Phytochemical analysis of some medicinal plants , 2011 .

[2]  F. Downes,et al.  Compendium of Methods for the Microbiological Examination of Foods , 2015 .

[3]  M. Agarwala,et al.  Phytochemical analysis of some medicinal plants , 2011 .

[4]  M. Kaneria,et al.  Indian nutraceutical plant leaves as a potential source of natural antimicrobial agents , 2011 .

[5]  S. Ibrahim,et al.  Antimicrobial herb and spice compounds in food. , 2010 .

[6]  A. Wisniewski,et al.  Chemical Composition and Antimicrobial Activity of Essential Oils of Eugenia chlorophylla (Myrtaceae) , 2008 .

[7]  W. E. Pinchak,et al.  Comparative antimicrobial activity of tannin extracts from perennial plants on mastitis pathogens , 2008 .

[8]  V. George,et al.  Chemical Constituents and Antibacterial Activity of the Leaf Oil of Cinnamomum chemungianum Mohan et Henry , 2007 .

[9]  K. Mena,et al.  Isolation of Antibiotic-Resistant Bacteria from the Air Plume Downwind of a Swine Confined or Concentrated Animal Feeding Operation , 2006, Environmental health perspectives.

[10]  W. Gritsanapan,et al.  Antimicrobial effects of Thai medicinal plants against acne-inducing bacteria. , 2005, Journal of ethnopharmacology.

[11]  C. Nakamura,et al.  An evaluation of antibacterial activities of Psidium guajava (L.) , 2005 .

[12]  K. Matalka,et al.  The antimicrobial activities of Psidium guajava and Juglans regia leaf extracts to acne-developing organisms. , 2005, The American journal of Chinese medicine.

[13]  A. Ulubelen Cardioactive and antibacterial terpenoids from some Salvia species. , 2003, Phytochemistry.

[14]  S. Begum,et al.  Two New Triterpenoids from the Fresh Leaves of Psidium guajava , 2002, Planta medica.

[15]  Mustafa Demirci,et al.  Antimicrobial activity and chemical composition of some essential oils , 2002, Archives of pharmacal research.

[16]  T. Puckree,et al.  Anti-diarrhoeal evaluation of some medicinal plants used by Zulu traditional healers. , 2002, Journal of ethnopharmacology.

[17]  H. Akiyama,et al.  Antibacterial action of several tannins against Staphylococcus aureus. , 2001, The Journal of antimicrobial chemotherapy.

[18]  F. A. Gonçalves,et al.  Microbicidal effect of medicinal plant extracts (Psidium guajava Linn. and Carica papaya Linn.) upon bacteria isolated from fish muscle and known to induce diarrhea in children. , 2001, Revista do Instituto de Medicina Tropical de Sao Paulo.

[19]  G. G. Nascimento,et al.  Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria , 2000 .

[20]  S. O. Gnan,et al.  Inhibition of Staphylococcus aureus by aqueous Goiaba extracts. , 1999, Journal of ethnopharmacology.

[21]  Y. Wongkrajang,et al.  Anticough and antimicrobial activities of Psidium guajava Linn. leaf extract. , 1999, Journal of ethnopharmacology.

[22]  M. M. Cowan Plant Products as Antimicrobial Agents , 1999, Clinical Microbiology Reviews.

[23]  T. Yıldırmak,et al.  Multiple drug resistance patterns and plasmid profiles of non-typhi salmonellae in Turkey , 1998, Epidemiology and Infection.

[24]  R. Huebner,et al.  Nasopharyngeal carriage and antimicrobial resistance in isolates of Streptococcus pneumoniae and Haemophilus influenzae type b in children under 5 years of age in Botswana. , 1998, International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases.

[25]  J. van Staden,et al.  Antibacterial activity of South African plants used for medicinal purposes. , 1997, Journal of ethnopharmacology.

[26]  Masayoshi,et al.  Comparative study on the antibacterial activity of phytochemical flavanones against methicillin-resistant Staphylococcus aureus. , 1997, Journal of ethnopharmacology.

[27]  R. Vieira,et al.  COLIMETRIA DAS ÁGUAS MARINHAS DE FORTALEZA (CEARÁ BRASIL) E DETECÇÃO DE CEPAS DE Escherichia coli ENTEROINVASORA (EIEC) E ENTEROPATOGÊNICA CLÁSSICA (EPEC) , 1996 .

[28]  J. W. Alencar,et al.  Volatile Constituents of Psidium pohlianum Berg, and Psidium guyanensis Pers. , 1994 .

[29]  A. Cáceres,et al.  Plants used in Guatemala for the treatment of gastrointestinal disorders. 3. Confirmation of activity against enterobacteria of 16 plants. , 1993, Journal of ethnopharmacology.

[30]  G. Lutterodt,et al.  Inhibition of Microlax-induced experimental diarrhoea with narcotic-like extracts of Psidium guajava leaf in rats. , 1992, Journal of ethnopharmacology.

[31]  Takashi Tanaka,et al.  Tannins and Related Compounds. CXVI. Six New Complex Tannins, Guajavins, Psidinins and Psiguavin from the Bark of Psidium guajava L. , 1992 .

[32]  C. Vanderzant,et al.  Compendium of Methods for the Microbiological Examination of Foods , 1992 .

[33]  A. Scalbert Antimicrobial properties of tannins , 1991 .

[34]  E. Hofer,et al.  Resistência antimicrobiana de Salmonella agona oriundas de várias regiões do Brasil , 1986 .

[35]  G. L. Cruz,et al.  Dicionário das plantas úteis do Brasil , 1979 .

[36]  A. Bauer,et al.  Antibiotic susceptibility testing by a standardized single disk method. , 1966, American journal of clinical pathology.

[37]  L. W. Parr COLIFORM BACTERIA , 1939, Bacteriological reviews.