Exploiting the mosaic structure of trans-acyltransferase polyketide synthases for natural product discovery and pathway dissection

[1]  R. Borriss,et al.  Biosynthesis of the antibiotic bacillaene, the product of a giant polyketide synthase complex of the trans-AT family. , 2007, Angewandte Chemie.

[2]  R. Borriss,et al.  Macrolactin is the polyketide biosynthesis product of the pks2 cluster of Bacillus amyloliquefaciens FZB42. , 2007, Journal of natural products.

[3]  S. Kitani,et al.  Characterization of biosynthetic gene cluster for the production of virginiamycin M, a streptogramin type A antibiotic, in Streptomyces virginiae. , 2007, Gene.

[4]  H. Gross Strategies to unravel the function of orphan biosynthesis pathways: recent examples and future prospects , 2007, Applied Microbiology and Biotechnology.

[5]  Sinisa Hrvatin,et al.  Widespread Occurrence and Genomic Context of Unusually Small Polyketide Synthase Genes in Microbial Consortia Associated with Marine Sponges , 2007, Applied and Environmental Microbiology.

[6]  Sinisa Hrvatin,et al.  Rapid isolation of rare clones from highly complex DNA libraries by PCR analysis of liquid gel pools. , 2007, Journal of microbiological methods.

[7]  M. Fischbach,et al.  The identification of bacillaene, the product of the PksX megacomplex in Bacillus subtilis , 2007, Proceedings of the National Academy of Sciences.

[8]  C. Hertweck,et al.  A Gene Cluster Encoding Rhizoxin Biosynthesis in “Burkholderia rhizoxina”, the Bacterial Endosymbiont of the Fungus Rhizopus microsporus , 2007, Chembiochem : a European journal of chemical biology.

[9]  Jacques Ravel,et al.  Natural combinatorial peptide libraries in cyanobacterial symbionts of marine ascidians , 2006, Nature chemical biology.

[10]  Thomas Börner,et al.  Natural Biocombinatorics in the Polyketide Synthase Genes of the Actinobacterium Streptomyces avermitilis , 2006, PLoS Comput. Biol..

[11]  Rolf Müller,et al.  Myxovirescin A Biosynthesis is Directed by Hybrid Polyketide Synthases/Nonribosomal Peptide Synthetase, 3‐Hydroxy‐3‐Methylglutaryl–CoA Synthases, and trans‐Acting Acyltransferases , 2006, Chembiochem : a European journal of chemical biology.

[12]  Chu-Young Kim,et al.  The 2.7-Å crystal structure of a 194-kDa homodimeric fragment of the 6-deoxyerythronolide B synthase , 2006 .

[13]  M. Fischbach,et al.  Assembly-line enzymology for polyketide and nonribosomal Peptide antibiotics: logic, machinery, and mechanisms. , 2006, Chemical reviews.

[14]  P. Dorrestein,et al.  Convergence of isoprene and polyketide biosynthetic machinery: isoprenyl-S-carrier proteins in the pksX pathway of Bacillus subtilis. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[15]  G. Gottschalk,et al.  Structural and Functional Characterization of Three Polyketide Synthase Gene Clusters in Bacillus amyloliquefaciens FZB 42 , 2006, Journal of bacteriology.

[16]  R. Müller,et al.  Identification and analysis of the chivosazol biosynthetic gene cluster from the myxobacterial model strain Sorangium cellulosum So ce56. , 2006, Journal of biotechnology.

[17]  C. Hertweck,et al.  Pathogenic fungus harbours endosymbiotic bacteria for toxin production , 2005, Nature.

[18]  Rolf Müller,et al.  Evolutionary implications of bacterial polyketide synthases. , 2005, Molecular biology and evolution.

[19]  Rolf Müller,et al.  Formation of novel secondary metabolites by bacterial multimodular assembly lines: deviations from textbook biosynthetic logic. , 2005, Current opinion in chemical biology.

[20]  T. Vogel,et al.  Type I Polyketide Synthases May Have Evolved Through Horizontal Gene Transfer , 2005, Journal of Molecular Evolution.

[21]  David Posada,et al.  ProtTest: selection of best-fit models of protein evolution , 2005, Bioinform..

[22]  Emmanuel Zazopoulos,et al.  Microbial genomics as a guide to drug discovery and structural elucidation: ECO-02301, a novel antifungal agent, as an example. , 2005, Journal of natural products.

[23]  Matthias Platzer,et al.  Antitumor polyketide biosynthesis by an uncultivated bacterial symbiont of the marine sponge Theonella swinhoei. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[24]  S. Matsunaga,et al.  Targeting modular polyketide synthases with iteratively acting acyltransferases from metagenomes of uncultured bacterial consortia. , 2004, Environmental microbiology.

[25]  M. Platzer,et al.  Unprecedented Diversity of Catalytic Domains in the First Four Modules of the Putative Pederin Polyketide Synthase , 2004, Chembiochem : a European journal of chemical biology.

[26]  S. Kroken,et al.  Phylogenomic analysis of type I polyketide synthase genes in pathogenic and saprobic ascomycetes , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[27]  K. Hiratsu,et al.  The large linear plasmid pSLA2‐L of Streptomyces rochei has an unusually condensed gene organization for secondary metabolism , 2003, Molecular microbiology.

[28]  Christopher M Thomas,et al.  Characterization of the mupirocin biosynthesis gene cluster from Pseudomonas fluorescens NCIMB 10586. , 2003, Chemistry & biology.

[29]  B. Neilan,et al.  Evolutionary Affiliations Within the Superfamily of Ketosynthases Reflect Complex Pathway Associations , 2003, Journal of Molecular Evolution.

[30]  Robert Finking,et al.  Biosynthesis of nonribosomal peptides , 2003 .

[31]  B. Shen,et al.  Type I polyketide synthase requiring a discrete acyltransferase for polyketide biosynthesis , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[32]  Brian O. Bachmann,et al.  A genomics-guided approach for discovering and expressing cryptic metabolic pathways , 2003, Nature Biotechnology.

[33]  Jörn Piel,et al.  A polyketide synthase-peptide synthetase gene cluster from an uncultured bacterial symbiont of Paederus beetles , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[34]  John P. Huelsenbeck,et al.  MRBAYES: Bayesian inference of phylogenetic trees , 2001, Bioinform..

[35]  S. Whelan,et al.  A general empirical model of protein evolution derived from multiple protein families using a maximum-likelihood approach. , 2001, Molecular biology and evolution.

[36]  B A Pfeifer,et al.  Biosynthesis of Complex Polyketides in a Metabolically Engineered Strain of E. coli , 2001, Science.

[37]  E. Mori,et al.  Identification of two polyketide synthase gene clusters on the linear plasmid pSLA2-L in Streptomyces rochei. , 2000, Gene.

[38]  G. Challis,et al.  Predictive, structure-based model of amino acid recognition by nonribosomal peptide synthetase adenylation domains. , 2000, Chemistry & biology.

[39]  R. Heath,et al.  The 1.8 A crystal structure and active-site architecture of beta-ketoacyl-acyl carrier protein synthase III (FabH) from escherichia coli. , 2000, Structure.

[40]  T. Stachelhaus,et al.  The specificity-conferring code of adenylation domains in nonribosomal peptide synthetases. , 1999, Chemistry & biology.

[41]  E. Ron,et al.  The first gene in the biosynthesis of the polyketide antibiotic TA of Myxococcus xanthus codes for a unique PKS module coupled to a peptide synthetase. , 1999, Journal of molecular biology.

[42]  J. Thompson,et al.  The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. , 1997, Nucleic acids research.

[43]  D. Hopwood,et al.  Genetic Contributions to Understanding Polyketide Synthases. , 1997, Chemical reviews.

[44]  D. Pirnik,et al.  Bacillaene, a novel inhibitor of procaryotic protein synthesis produced by Bacillus subtilis: production, taxonomy, isolation, physico-chemical characterization and biological activity. , 1995, The Journal of antibiotics.

[45]  A. Albertini,et al.  Sequence around the 159 ' region of the Bacillus subtilis genome : the pksX locus spans 33 . 6 kb , 2008 .

[46]  Mark Hildebrand,et al.  Identification of the putative bryostatin polyketide synthase gene cluster from "Candidatus Endobugula sertula", the uncultivated microbial symbiont of the marine bryozoan Bugula neritina. , 2007, Journal of natural products.

[47]  Chu-Young Kim,et al.  The 2.7-Angstrom crystal structure of a 194-kDa homodimeric fragment of the 6-deoxyerythronolide B synthase. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[48]  J. V. Lopez Naturally mosaic operons for secondary metabolite biosynthesis: variability and putative horizontal transfer of discrete catalytic domains of the epothilone polyketide synthase locus , 2003, Molecular Genetics and Genomics.

[49]  C. Carlson,et al.  The biology of natural transformation. , 1986, Annual review of microbiology.