Phase modulation mode of scanning ion conductance microscopy

This Letter reports a phase modulation (PM) mode of scanning ion conductance microscopy. In this mode, an AC current is directly generated by an AC voltage between the electrodes. The portion of the AC current in phase with the AC voltage, which is the current through the resistance path, is modulated by the tip-sample distance. It can be used as the input of feedback control to drive the scanner in Z direction. The PM mode, taking the advantages of both DC mode and traditional AC mode, is less prone to electronic noise and DC drift but maintains high scanning speed. The effectiveness of the PM mode has been proven by experiments.

[1]  Y. Shao,et al.  Probing the structure of a water/nitrobenzene interface by scanning ion conductance microscopy , 2011 .

[2]  Tomoyuki Yasukawa,et al.  Topographic imaging of convoluted surface of live cells by scanning ion conductance microscopy in a standing approach mode. , 2010, Physical chemistry chemical physics : PCCP.

[3]  D. Klenerman,et al.  A novel light source for SICM–SNOM of living cells , 2003, Journal of microscopy.

[4]  Patrick Happel,et al.  Scanning Ion Conductance Microscopy for Studying Biological Samples , 2012, Sensors.

[5]  Sang-Joon Cho,et al.  Scanning ion conductance microscopy studies of amyloid fibrils at nanoscale. , 2012, Nanoscale.

[6]  Yuri E. Korchev,et al.  Functional localization of single active ion channels on the surface of a living cell , 2000, Nature Cell Biology.

[7]  P. Hansma,et al.  The scanning ion-conductance microscope. , 1989, Science.

[8]  M J Lab,et al.  Scanning ion conductance microscopy of living cells. , 1997, Biophysical journal.

[9]  Albert L. Lipson,et al.  Nanoscale In Situ Characterization of Li‐ion Battery Electrochemistry Via Scanning Ion Conductance Microscopy , 2011, Advanced materials.

[10]  Z. Shao,et al.  Characterization of AC mode scanning ion-conductance microscopy. , 2001, Ultramicroscopy.

[11]  D. Klenerman,et al.  Simultaneous measurement of Ca2+ and cellular dynamics: combined scanning ion conductance and optical microscopy to study contracting cardiac myocytes. , 2001, Biophysical journal.

[12]  T. Schäffer,et al.  Mapping the mechanical stiffness of live cells with the scanning ion conductance microscope , 2013 .

[13]  Chao Li,et al.  High resolution imaging using scanning ion conductance microscopy with improved distance feedback control , 2008 .

[14]  D. Klenerman,et al.  Scanning ion conductance microscopy reveals how a functional renal epithelial monolayer maintains its integrity. , 2005, Kidney international.

[15]  D. Klenerman,et al.  Nanoscale live-cell imaging using hopping probe ion conductance microscopy , 2009, Nature Methods.