Synthesis of 3H labeled dihydrorotenone

The catalytic tritiation of rotenone results in the preparation of two products, the expected tritiated 6′,7′-dihydrorotenone ([3H]DHR) and tritiated 6′,7′-dihydrorotenol ([3H]DHR-ol). The ratio of [3H]DHR to [3H]DHR-ol is 9 to 1. Reversed-phase HPLC provided the purified [3H]DHR and [3H]DHR-ol with estimated specific activities of 45 and 60 Ci/mmol, respectively. © 1997 John Wiley & Sons, Ltd.

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