Sialic acid receptor specificity on erythrocytes affects detection of antibody to avian influenza haemagglutinin

Haemagglutination‐inhibition tests (HI) are used to detect increases in influenza antibody in serum. However, they are relatively insensitive for the detection of human antibody responses to avian haemagglutinin, even in the presence of high titres of neutralising antibody after confirmed infection or vaccination. Human influenza viruses bind preferentially sialic acid containing N‐acetylneuraminic acid α2,6‐galactose (SAα2,6Gal) linkages while avian and equine viruses bind preferentially those containing N‐acetylneuraminic acid α2,3‐galactose (SAα2,3Gal) linkages. Increasing the proportion of SAα2,3Gal linkages on the erythrocytes used, by enzymatic modification or change of species, improves the ability of erythrocytes to bind to avian influenza strains and thereby improves the sensitivity of detection of antibody to avian and equine HA in a range of mammalian and human sera using HI tests. J. Med. Virol. 70:391–398, 2003. © 2003 Wiley‐Liss, Inc.

[1]  R. Webster,et al.  Replication of avian influenza viruses in humans , 2005, Archives of Virology.

[2]  G. Palladino,et al.  Serological evidence of human infections with avian influenza viruses , 2005, Archives of Virology.

[3]  Maria Zambon,et al.  Boosting immunity to influenza H5N1 with MF59-adjuvanted H5N3 A/Duck/Singapore/97 vaccine in a primed human population. , 2003, Vaccine.

[4]  N. Escriou,et al.  Hemagglutinin residues of recent human A(H3N2) influenza viruses that contribute to the inability to agglutinate chicken erythrocytes. , 2001, Virology.

[5]  J. Skehel,et al.  X-ray structures of H5 avian and H9 swine influenza virus hemagglutinins bound to avian and human receptor analogs , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[6]  M. Zambon,et al.  Safety and antigenicity of non-adjuvanted and MF59-adjuvanted influenza A/Duck/Singapore/97 (H5N3) vaccine: a randomised trial of two potential vaccines against H5N1 influenza , 2001, The Lancet.

[7]  E. Nobusawa,et al.  Change in receptor-binding specificity of recent human influenza A viruses (H3N2): a single amino acid change in hemagglutinin altered its recognition of sialyloligosaccharides. , 2000, Virology.

[8]  Keiji Fukuda,et al.  Detection of Antibody to Avian Influenza A (H5N1) Virus in Human Serum by Using a Combination of Serologic Assays , 1999, Journal of Clinical Microbiology.

[9]  R. Webster,et al.  Human influenza A H5N1 virus related to a highly pathogenic avian influenza virus , 1998, The Lancet.

[10]  L. Mitnaul,et al.  Receptor specificity of influenza A viruses correlates with the agglutination of erythrocytes from different animal species. , 1997, Virology.

[11]  R. Webster,et al.  Receptor specificity in human, avian, and equine H2 and H3 influenza virus isolates. , 1994, Virology.

[12]  I. Wilson,et al.  Single amino acid substitutions in influenza haemagglutinin change receptor binding specificity , 1983, Nature.

[13]  J. Paulson,et al.  Receptor determinants of human and animal influenza virus isolates: differences in receptor specificity of the H3 hemagglutinin based on species of origin. , 1983, Virology.

[14]  R. Webster,et al.  Failure to Detect Hemagglutination-Inhibiting Antibodies with Intact Avian Influenza Virions , 1982, Infection and immunity.