Cultivation of microorganisms in an air–solid fluidized bed fermentor with agitators

The productivity of a cell mass of Saccharomyces cerevisiae and enzymes of Eupenicillium javanicum increased by cultivation in anair–solid fluidized bed fermentor with agitators. The usefulness of the apparatus for the fluidized bed culture was verified. The productivity of amyiase and protease of the fungus by fluidized bed culture was twice as high as that by stationary culture, considering the dry weight of cells and the enzyme activity. Physiological properties of yeast cells were changed buy the fluidized bed culture; there was a decrease in the cell size of yeast and the changes to the aerobic properties of the yeast cells resulting from excessive supply of oxygen with a high flowrate of air.