Elongation Pathway for a-Linolenic Acid Synthesis in Spinach Leaves

Leaf slices from spinach exhibited considerable variation in their incor-poration of I14Cibicarbonate and II-14Clacetate into fatty acids. Reductive ozonolysis studies indicated that aD the 14C-labeled fatty acids were synthesized de now; no in vivo evidence was found for the previously proposed elongation of hexadecatrienoate to a-linolenate.

[1]  J. Ohlrogge,et al.  Subcellular localization of acyl carrier protein in leaf protoplasts of Spinacia oleracea. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[2]  D. Murphy,et al.  Light-dependent Induction of Polyunsaturated Fatty Acid Biosynthesis in Greening Cucumber Cotyledons. , 1979, Plant physiology.

[3]  P. Stumpf,et al.  Fat metabolism in higher plants. The elongation of saturated and unsaturated acyl-CoAs by a stromal system from isolated spinach chloroplasts. , 1978, Archives of biochemistry and biophysics.

[4]  C. J. Bedord,et al.  α-linolenic acid biosynthesis in Cyanidium caldarium , 1978 .

[5]  F. Quackenbush,et al.  Alternate pathways of linolenic acid biosynthesis in growing cultures of Penicillium chrysogenum. , 1974, Archives of biochemistry and biophysics.

[6]  B. Jacobson,et al.  The elongation of medium chain trienoic acids to -linolenic acid by a spinach chloroplast stroma system. , 1973, Biochemical and biophysical research communications.

[7]  S. D. Fowler,et al.  Methodology for the separation o plant lipids and application to spinach leaf and chloroplast lamellae , 1966 .

[8]  R. Stein,et al.  Structure determination of methyl esters of unsaturated fatty acids by gas-liquid chromatography of the aldehydes formed by triphenyl phosphine reduction of the ozonides , 1962 .

[9]  Ma Yu.  The biosynthesis of polyunsaturated fatty acids , 2002 .

[10]  E. Mercer,et al.  Recent Advances in the Chemistry and Biochemistry of Plant Lipids , 1977 .

[11]  B. Jacobson,et al.  In vivo biosynthesis of -linolenic acid in plants. , 1973, Biochemical and Biophysical Research Communications - BBRC.

[12]  A. T. James,et al.  Linoleic and alpha-linolenic acid biosynthesis in plant leaves and green alga. , 1965, Biochimica et Biophysica Acta.