Detection of biomolecules in complex media using surface plasmon resonance sensors

Detection of multiple biologically relevant molecules was accomplished at sub-ng/mL levels in highly fouling media using fiber- optic based surface plasmon resonance sensors. Myocardial infarction markers, myoglobin and cTnI, were quantified in full serum with limits of detection below 1 ng/mL. Biologically relevant levels are between 15-30 ng/mL and 1-5 ng/mL for myoglobin and cTnI respectively. Cytokines involved in chronic wound healing, Interleukin 1, Interleukin 6, and tumor necrosis factor α, were detected at around 1 ng/mL in cell culture media. Preliminary results in monitoring these cytokines in cell cultures expressing the cytokines were obtained. The protein diagnostic of spinal muscular atrophy, survival motor neuron protein, was quantified from cell lysate. To obtain such results in complex media, the sensor's stability to non-specific protein adsorption had to be optimized. A layer of the N-hydroxysuccinimide ester of 16-mercaptohexadecanoic acid is attached to the sensor. This layer optimizes the antibody attachment to the sensor while minimizing the non-specific signal from serum proteins.

[1]  Jean-Francois Masson,et al.  Quantification of cytokines involved in wound healing using surface plasmon resonance. , 2005, Analytical chemistry.

[2]  R B Schasfoort,et al.  An immunosensor for syphilis screening based on surface plasmon resonance. , 1993, Biosensors & bioelectronics.

[3]  D C Cullen,et al.  Covalent coupling of immunoglobulin G to a poly(vinyl)alcohol-poly(acrylic acid) graft polymer as a method for fabricating the interfacial-recognition layer of a surface plasmon resonance immunosensor. , 1998, Biosensors & bioelectronics.

[4]  Renjun Pei,et al.  Enhanced surface plasmon resonance immunoassay for human complement factor 4 , 2002 .

[5]  Jean-Francois Masson,et al.  Monitoring of recombinant survival motor neuron protein using fiber-optic surface plasmon resonance. , 2004, The Analyst.

[6]  Jing-Min Hwang,et al.  Development of an immunosensor for human ferritin, a nonspecific tumor marker, based on surface plasmon resonance. , 2004, Biosensors & bioelectronics.

[7]  D. Mytych,et al.  Validation parameters for a novel biosensor assay which simultaneously measures serum concentrations of a humanized monoclonal antibody and detects induced antibodies. , 1997, Journal of immunological methods.

[8]  Jean-Francois Masson,et al.  Preparation of analyte-sensitive polymeric supports for biochemical sensors. , 2004, Talanta.

[9]  Daniel Mytych,et al.  Validation of the BIACORE 3000 platform for detection of antibodies against erythropoietic agents in human serum samples , 2003, Current medical research and opinion.

[10]  Peter Schuck,et al.  Surface plasmon resonance-based competition assay to assess the sera reactivity of variants of humanized antibodies. , 2002, Journal of immunological methods.

[11]  M Annette Johansson,et al.  Matrix effects in immunobiosensor determination of clenbuterol in urine and serum. , 2004, The Analyst.

[12]  I. Haro,et al.  Assessment of synthetic peptides for hepatitis A diagnosis using biosensor technology. , 2000, JIM - Journal of Immunological Methods.

[13]  Fan Yang,et al.  Real-time immunoassay of ferritin using surface plasmon resonance biosensor. , 2003, Talanta.

[14]  G. Morris.  Epitope mapping protocols , 1996 .

[15]  T. Lindahl,et al.  Immobilized chicken antibodies improve the detection of serum antigens with surface plasmon resonance (SPR). , 1998, Biosensors & bioelectronics.

[16]  Emi Hifumi,et al.  Elimination of Ingredients Effect to Improve the Detection of Anti HIV-1 p24 Antibody in Human Serum Using SPR Apparatus , 2002, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry.

[17]  Karl S. Booksh,et al.  Tuning Dynamic Range and Sensitivity of White-Light, Multimode, Fiber-Optic Surface Plasmon Resonance Sensors , 1999 .

[18]  G Zhu,et al.  Quantitation of basic fibroblast growth factor by immunoassay using BIAcore 2000. , 2000, Journal of pharmaceutical and biomedical analysis.

[19]  Howard H. Weetall,et al.  Immobilized enzymes, antigens, antibodies, and peptides : preparation and characterization , 1975 .

[20]  Lars Johnsson,et al.  Reduction of Sample Matrix Effects - The Analysis of Benzimidazole Residues in Serum by Immunobiosensor , 2002 .

[21]  Tina M. Battaglia,et al.  Biocompatible polymers for antibody support on gold surfaces. , 2005, Talanta.

[22]  Marcus Andersson,et al.  Methods for research on immune complement activation on modified sensor surfaces , 2003 .

[23]  J. Köhl,et al.  Selection of phage-displayed anti-guinea pig C5 or C5a antibodies and their application in xenotransplantation. , 1999, Molecular immunology.

[24]  T. Chinowsky,et al.  Quantitative interpretation of the response of surface plasmon resonance sensors to adsorbed films , 1998 .

[25]  J. Attridge,et al.  Sensitivity enhancement of optical immunosensors by the use of a surface plasmon resonance fluoroimmunoassay. , 1991, Biosensors & bioelectronics.

[26]  S. Kubitschko,et al.  Sensitivity enhancement of optical immunosensors with nanoparticles. , 1997, Analytical biochemistry.

[27]  J. Homola,et al.  Surface plasmon resonance biosensor based on integrated optical waveguide , 2001 .

[28]  M. Stacey,et al.  Levels of Tumor Necrosis Factor-α (TNF-α) and Soluble TNF Receptors in Chronic Venous Leg Ulcers – Correlations to Healing Status , 1998 .

[29]  R. Pei,et al.  Real-time immunoassay of antibody activity in serum by surface plasmon resonance biosensor. , 2000, Talanta.

[30]  F. van Knapen,et al.  Surface plasmon resonance (BIACORE) detection of serum antibodies against Salmonella enteritidis and Salmonella typhimurium. , 2002, Journal of immunological methods.

[31]  J. Andrade.  Surface and Interfacial Aspects of Biomedical Polymers , 1985 .

[32]  K. Gaus,et al.  Surface plasmon resonance sensor for heparin measurements in blood plasma. , 1998, Biosensors & bioelectronics.

[33]  N. Latov,et al.  A surface plasmon resonance biosensor assay for measurement of anti-GM(1) antibodies in neuropathy. , 2001, Neurology.