A novel glucose sensor system with Au nanoparticles based on microdialysis and coenzymes for continuous glucose monitoring

Abstract A novel glucose sensor based on microdialysis technique has been developed for continuous glucose monitoring. It includes: (1) The microdialysis sampling and interval perfusion system, it can prevent electrode from being fouled and it also provides enough time for glucose molecule traversing the microdialysis membrane to establish a dynamic balance, and the sensor can be recovered well, too. (2) A “sandwich” structure glucose sensor based on an aqueous glucose oxidase (GOD) and catalase solution, it is easy to replace the inactive enzyme solution. Au interdigital array (IDA) microelectrodes and microgrooves were fabricated on the silicon wafer for electrochemistry determinations. (3) Continuous glucose monitoring system. The results of in vitro experiments show this glucose sensor has short response time, high sensitivity and good linearity. To improve the stability of liquid enzyme, aqueous colloidal gold nanoparticles was mixed with coenzymes solution. The result of experiments shows the repeatability of sensor was improved, and the sensitivity of sensor was enhanced. To the best of our knowledge, this is the first demonstration that aqueous colloidal gold nanoparticles enhance the activity of aqueous enzymes.

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